Biography

Dr. George Patterson received a bachelor's degree in biology from the University of North Alabama in 1992 where he specialized in research on the reproductive cycle of a freshwater mussel, Cyclonais tuberculata. Accepted into the Interdisciplinary Graduate Program at Vanderbilt University School of Medicine in 1993, he joined the lab of Dr.David Piston in the Department of Molecular Physiology and was awarded a Ph.D. in 1999 for research on glucose metabolism in pancreatic islets of Langerhans and quantitative fluorescence imaging of proteins. He subsequently accepted a post-doctoral position in the lab of Jennifer Lippincott-Schwartz in the Cell Biology and Metabolism Branch of the National Institute of Child Health and Human Development at NIH where he developed reagents and refined fluorescence imaging techniques for use in research on secretory protein trafficking. Four years later, he was promoted to a staff scientist position at the NIH as he continued his work on protein trafficking and also worked in collaboration with Eric Betzig and Harald Hess of Howard Hughes Medical Institute Janelia Farms in development of new fluorescence imaging techniques. Also at that time, his other collaborative work with Vladislav Verkhusha of the Albert Einstein School of Medicine focused on development of novel fluorescence imaging reagents. In October 2009, Dr. Patterson accepted a position as Chief of the Biophotonics Section at the NIBIB where he continues his research on fluorescent protein development for use in cell biology applications.

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Publications

Subach FV, Patterson GH, Manley SM, Gillette JM, Lippincott-Schwartz J, and Verkhusha VV. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods, 6:153-159, 2009.

Patterson GH, Hirschberg K, Polishchuk RS, Gerlich D, Phair RD, and Lippincott-Schwartz J. Transport through the Golgi apparatus by rapid partitioning within a two-phase membrane system. Cell, 133:1055-1067, 2008.

Manley S, Gillette JM, Patterson GH, Shroff H, Hess HF, Betzig E, and Lippincott-Schwartz J. Mapping single molecule diffusion in living cells with Photoactivated Localization Microscopy. Nature Methods, 5:155-157, 2008.

Lippincott-Schwartz J, and Patterson GH. Fluorescent proteins for photoactivation experiments. Methods in Cell Biology, 85:45-61, 2008.

Kanaani J, Patterson GH, Schaufele F, Lippincott-Schwartz J, and Baekkeskov S. A palmitoylation cycle dynamically regulates partitioning of the GABA-synthesizing enzyme GAD65 between ER/Golgi and post-Golgi membranes. Journal of Cell Science, 121:437-449, 2007.

Shaner NC, Patterson GH, and Davidson MW. Advances in fluorescent protein technology. Journal of Cell Science, 120:4247-4260, 2007.

Patterson, GH. Fluorescent proteins for cell biology. Methods in Molecular Biology, 411:47-80, 2007.

Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, Bonifacino JS, Davidson MW, Lippincott-Schwartz J, and Hess HF. Imaging intracellular fluorescent proteins at nanometer resolution. Science, 313:1642-1645, 2006.

Chen Y, MacDonald PJ, Skinner JP, Patterson GH, and Müller JD. Probing nucleocytoplasmic transport by two-photon activation of PA-GFP. Microscopy Research and Technique, 69:220-226, 2006.

Rossman JS, Stoicheva NG, Langel FD, Patterson GH, Lippincott-Schwartz J, and Schaefer BC. POLKADOTS are foci of functional interactions in T-cell receptor-mediated signaling to NF-kB. Molecular Biology of the Cell, 17:2166-2176, 2006.

Hodges M, Yikilmaz E, Patterson G, Kasvosve I, Rouault TA, Gordeuk VR, and Loyevsky M. An iron regulatory-like protein expressed in Palsmodium falciparium displays aconitase activity. Molecular & Biochemical Parasitology, 143:29-38, 2005.

Patterson GH. A new harvest of fluorescent proteins. Nature Biotechnology, 22: 1524-1525, 2004.

Patterson GH and Lippincott-Schwartz J. Development of a photoactivatable fluorescent protein from Aequorea victoria GFP. Proceedings of SPIE, 5329:13-22, 2004.

Patterson GH and Lippincott-Schwartz J. Selective photolabeling of proteins using photoactivatable GFP. Methods, 32:445-450, 2004.

Lippincott-Schwartz J and Patterson GH. Development and use of fluorescent protein markers in living cells. Science, 300:87-91, 2003.

Patterson GH and Lippincott-Schwartz J. A photoactivatable GFP for selective photolabeling of proteins and cells. Science, 297:1873-1877, 2002.

Patterson GH, Day RN, and Piston DW. Fluorescent protein spectra. Journal of Cell Science, 114:837-838, 2001.

Patterson GH, Piston DW, and Barisas BG. Förster distances between green fluorescent protein pairs. Analytical Biochemistry, 284:438-440, 2000.

Patterson GH, Knobel SM, Arkhammar P, Thastrup O, and Piston DW. Separation of the glucose-stimulated cytoplasmic and mitochondrial NAD(P)H responses in pancreatic islet b cells. Proceedings of the National Academy of Science, 97:5203-5207, 2000.

Patterson GH and Piston DW. Photobleaching in two-photon excitation microscopy. Biophysical Journal, 78:2159-2162, 2000.

Miller III DM, Desai NS, Hardin DC, Piston DW, Patterson GH, Fleenor J, Xu S, and Fire A. Two-color GFP expression system for C. elegans. Biotechniques, 26:914-921, 1999.

Piston DW, Patterson GH, and Knobel SM. Quantitative imaging of the green fluorescent protein (GFP). Methods in Cell Biology, 58:31-48, 1998.

Patterson GH, Schroeder SC, Bai Y, Weil PA, Piston DW. Quantitative imaging of TATA-binding protein in living yeast cells. Yeast, 14:813-825, 1998.

Lybarger L, Dempsey D, Patterson GH, Piston DW, Kain SR, and Chervenak R. Dual-color flow cytometric detection of fluorescent proteins using single-laser (488-nm) excitation. Cytometry, 31:147-152, 1998.

Yang T-T, Sinai P, Green G, Kitts PA, Chen Y-T, Lybarger L, Chervenak R, Patterson GH, Piston DW, and Kain SR. Improved fluorescence and dual color detection with enhanced blue and green variants of the green fluorescent protein. J. Biol. Chem, 273:(14)8212-8216, 1998.

Patterson GH, Knobel SM, Sharif WD, Kain SR, and Piston DW. Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy. Biophysical Journal, 73:2782-2790, 1997.

Haggerty TM, Garner JT, Patterson GH, and Jones Jr. LC. A quantitative assessment of the reproductive biology of Cyclonais tuberculata (Bivalvia: Unionidae). Canadian Journal of Zoology, 73:83-88, 1995.

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