About the videos on this page (below): Times are indicated in hours: minutes:seconds; the embryos were imaged volumetrically every 2s until hatching. All movies were acquired by Yicong Wu and Alireza Ghitani in collaboration with the Colon-Ramos (Yale) and Bao (MSKCC) labs. Above: Labeling Cell Nuclei in a Developing C. Elegans Embryo Above: Glow Worm: Green Fluorescent Proteins light up C. elegans Above:Seeking Clues to Human Brain Development by Observing C. elegans

Section on High Resolution Optical Imaging

Contents

• Expertise
• Meet the Staff
• Recent Publications and related information

Expertise

The NIBIB Section on High Resolution Optical Imaging (HROI) develops novel technologies for studying biological processes at unprecedented speed and resolution. Research interests and ongoing projects include:

• Furthering development of superresolution optical imaging techniques, particularly 3-D photoactivated localization microscopy (PALM)
• Identification and screening of new photoactivable proteins and dyes for use in PALM or conventional imaging
• Synthesis of novel non-diffracting light sheets and subsequent application to selective plane illumination microscopy (SPIM)
• Improving multiphoton temporal focusing for high-speed imaging of cells and embryos
• Development of high-speed, high-resolution THz microscope for diagnosing skin cancer and imaging biological surfaces
• Construction of a 4-dimensional digital atlas of neuronal development in C. Elegans in collaboration with researchers at Yale University and Memorial Sloan-Kettering Cancer Center

Meet the Staff

Hari Shroff, Ph.D. - Chief and Investigator

Yicong Wu, Ph.D. - Research Fellow

Kelsey Temprine, B.S. - Postbaccalaureate IRTA

Andrew York, Ph.D. - Research Fellow

Peter W. Winter, Ph.D. - IRTA Postdoctoral Fellow

Recent Publications

Wu, Y., Ghitani, A., Christensen, R., Santella, A., Du, Z., Rondeau, G., Bao, Z., Colon-Ramos, D., Shroff, H. Inverted selective plane illumination microscopy (iSPIM) enables coupled cell identity lineaging and neurodevelopmental imaging in Caenorhabditis elegans. PNAS 108, 17708-17713 (2011).

Back to Top