Hari Shroff, Ph.D.
Chief and Investigator
Section on High Resolution Optical Imaging
Bldg. 13, Rm. G800
Dr. Hari Shroff received a B.S.E. in bioengineering from the University of
Washington in 2001, and under the supervision of Dr. Jan Liphardt, completed
his Ph.D. in biophysics at the Unversity of California at Berkeley in 2006
. He spent the next three years performing postdoctoral research under the
mentorship of Eric Betzig at the Howard Hughes Medical Institute's Janelia
Farm Research Campus where his research focused on development of photactivated
localization microscopy (PALM), an optical superresolution technique. Dr. Shroff
is now chief of NIBIB's section on high resolution optical imaging
laboratory, where he and his staff are developing new imaging tools for
application in biological and clinical research.
- Development of new imaging tools to study fast 3D cellular processes
- Super-resolution microscopy
- Cell motility
- Single molecule fluorescence
- Physical properties of highly-bent DNA molecules
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Wu, Y., Ghitani, A., Christensen, R., Santella, A., Du, Z., Rondeau, G., Bao, Z.,
Colon-Ramos, D., Shroff, H. Inverted selective plane illumination microscopy (iSPIM)
enables coupled cell identity lineaging and neurodevelopmental imaging in Caenorhabditis
elegans. PNAS 108, 17708-17713 (2011).
York A.G., Ghitani, A., Vaziri, A., Davidson M.W., Shroff, H. Confined activation
and subdiffractive localization enables whole-cell PALM with genetically expressed
probes. Nat. Methods 4: 327-333 (2011). Click
here to see the HROI Palm 3D code and software information web
Frost NA, Shroff H, Kong
H, Betzig E, Blanpied
TA. Single-molecule discrimination of discrete perisynaptic and distributed sites
of actin filament assembly within dendritic spines. Neuron, 67(1):86-99,
Greenfield D, McEvoy AL, Shroff H, Crooks GE, Wingreen NS, Betzig E, Liphardt J.
Self-Organization of the Escherichia coli Chemotaxis Network Imaged with Super-Resolution
Light Microscopy. PLoS Biology, 7:e1000137, 2009.
Vaziri A, Tang J, Shroff H, Shank CV. Multilayer Three-Dimensional Super-resolution
Imaging of Thick Samples. PNAS 105:20221-20226, 2008.
Ji N, Shroff H, Zhong H, Betzig E. Advances in the speed and resolution of light
microscopy. Current Opinion in Neurobiology, 18:605-616, 2008.
Shroff H, White H, Betzig E. Photoactivation localization microscopy (PALM) of adhesion
complexes. Current Protocols in Cell Biology, 4.21.1-4.21.27, 2008.
Shroff H, Galbraith, C.G., Galbraith, J.A., Betzig, E. Live-cell photoactivated
localization microscopy of nanoscale adhesion dynamics. Nat Methods, 5:417-423,
Manley S, Gillette JM, Patterson GH, Shroff H, Hess HF, Betzig E, Lippincott-Schwartz
J. High-density mapping of single-molecule trajectories with photoactivated localization
microscopy. Nat Methods, 5:155-157, 2008.
Shroff H, Sivak D, Siegel JJ, McEvoy A, Siu M, Spakowitz A, Geissler P, Liphardt
J. Optical measurement of mechanical forces inside short DNA loops. Biophys J,
Shroff H, Galbraith CG, Galbraith JA, White H, Gillette J, Olenych S, Davidson MW,
Betzig E. Dual-color superresolution imaging of genetically expressed probes within
individual adhesion complexes. PNAS, 104:20308-20313, 2007.
Pauzauskie PJ, Radenovic A, Trepagnier E, Shroff H, Yang P, Liphardt J. Optical
trapping and integration of nanowire assemblies in water. Nat Materials,
Shroff H, Reinhard BM, Siu M, Agarwal H, Spakowitz A, Liphardt J. Biocompatible
force sensor with optical readout and dimensions of 6 nm3. Nano Lett, 5:1509-1514,
Former Lab Members
Alireza Ghitani, B.S.
Mr. Ghitani received his Bachelor of Science degree in biology from George Mason
University and participated in the Postbaccalaureate IRTA program at NIBIB from
2010-2011. While in training at NIBIB, he worked with Caenorhabditis elegans
using modified microscopes to look for differentiated cells and their location to
Kelsey Temprine, B.S.
Ms. Kelsey Temprine received her Bachelor of Science degree from the University
of Texas at Austin and participated in the Postbaccalaureate IRTA program at NIBIB
from 2011-2012. While in training, she applied PALM to the study of bacterial sporulation
and helped to develop a new form of structured illumination microscopy. She is currently
a graduate student at the Memorial Sloan-Kettering Cancer Center.
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Last Updated On 07/16/2012