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Analytical Ultracentrifugation Support

Introduction

An introduction to modern analytical ultracentrifugation for the study of reversible protein-protein interactions is to appear in Cold Spring Harbor Laboratory Press, Protein-Protein Interactions: A Molecular Cloning Manual.

Download a preprint of the introduction (PDF)

Protocols

This work refers to detailed step-by-step protocol for conducting sedimentation velocity and sedimentation equilibrium experiments, which are available for download.

Protocols (PDF)

Tutorials

• Lamm equation solutions
• systematic noise analysis
• size-distribution analysis
• ls-g*(s) apparent sedimentation coefficient distributions
• van Holde-Weischet analysis
• compressibility of water and organic solvents
• sedimentation in dynamic self-forming density gradients

Analytical Ultracentrifugation Software

The following software applications for sedimentation velocity and sedimentation equilibrium analysis are available:

SEDFIT - a data analysis software for sedimentation velocity, sedimentation equilibrium, and dynamic light scattering. 

SEDPHAT - for the global analysis of data from multiple experiments (sedimentation velocity, sedimentation equilibrium, dynamic light scattering, isotherms).