Home > Research > NIBIB Intramural Labs > Laboratory of Bioengineering and Physical Science > LBPS Dynamics of Macromolecular Assembly > LBPS, Analytical Ultracentrifugation Support

E-mail this page

Analytical Ultracentrifugation - SEDPHAT

SEDPHAT is used for the global analysis of data from multiple experiments (sedimentation velocity, sedimentation equilibrium, dynamic light scattering, isotherms).

Download SEDPHAT

System requirements

• PC with at least 500 MHz processor and 200 MB RAM for equilibrium, but > 2 GHz processor and 500 MB RAM for global velocity analysis
• Windows NT, 95, 98, 2000, XP or later
• ~5 MB of harddisk space
• internet connection for access to on-line help

Installation

1) Note that this is software was created at the National Institutes of Health and carries the following disclaimer:

THIS SOFTWARE IS PROVIDED AS IS, AND WITH ALL FAULTS. THERE ARE NO WARRANTIES OF ANY KIND, EXPRESS, IMPLIED OR STATUTORY (INCLUDING, WITHOUT LIMITATION, TIMELINESS, TRUTHFULNESS, SEQUENCE, COMPLETENESS, ACCURACY, FREEDOM FROM INTERRUPTION). THERE ARE NO IMPLIED WARRANTIES ARISING FROM TRADE USAGE, COURSE OF DEALING, OR COURSE OF PERFORMANCE, OR THE IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. THERE IS NO WARRANTY THAT ANY SOFTWARE, INFORMATION, OR OUR EFFORTS WILL FULFILL ANY OF THE USERS PARTICULAR NEEDS OR PURPOSES

Please cite the software in publications and reference the method used.

2) Create a directory named "sedfit" on your computer's C: hard drive.

3) SEDPHAT is only a single executable sedphat.exe. Download this file simply by clicking on the following link, and store the files in the directory c:sedfit.

sedphat.exe (4218kb, this is the version 4.1b from March 2006)

For a brief list of changes from the previous version, see sedphatchanges.txt

The location of sedphat.exe should be c:sedfitsedphat.exe in order to establish the link to the export function of SEDFIT.

4) If it doesn't exist already, create a directory named "windows" on your C: hard drive. This directory will contain some configuration files once SEDPHAT is running.

5) You can also associate "*.sedphat" files with sedphat.exe [single right-click on sephat file, select "Open With", "Choose Program", "Browse", find sedphat.exe, and check "Always use the selected program..."]. This will allow you to simply double click on any sedphat file from within your file manager and start a new copy of SedphaT showing the saved analysis. Similarly, you can association "*.xp" files with sedphat.exe.

6) For getting started, visit the sites for Concepts for Getting Started, and the Tutorial for Getting Started (note this includes a description for how to associate '*.sedphat' files with sedphat.exe)

7) Remember: For storing any data, configuration files, or experiment files, DO NOT USE DIRECTORIES WITH WHITE-SPACE CHARACTERS (e.g. "My Documents", "XLA DATA" or the desktop). That will create errors when reloading.

8) Because SEDPHAT is evolving rapidly, I strongly recommend that you make a request to be placed on a user email list for updates, bug fixes, etc. If you would like to be on our user list, send an email to:

schuckp@mail.nih.gov with the subject line: SEDPHAT USER LIST

Resources

The approaches implemented in the software are described in detail in:

• Vistica J, Dam J, Balbo A, Yikilmaz E, Mariuzza RA, Rouault TA and Schuck P. Sedimentation equilibrium analysis of protein interactions with global implicit mass conservation constraints and systematic noise decomposition. Analytical Biochemistry 326:234-256, 2004.

• Schuck P. On the analysis of protein self-association by sedimentation velocity analytical ultracentrifugation. Analytical Biochemistry 320:104-124, 2003.