Biography

Dr. Hari Shroff received a B.S.E. in bioengineering from the University of Washington in 2001, and under the supervision of Dr. Jan Liphardt, completed his Ph.D. in biophysics at the Unversity of California at Berkeley in 2006 . He spent the next three years performing postdoctoral research under the mentorship of Eric Betzig at the Howard Hughes Medical Institute's Janelia Farm Research Campus where his research focused on development of photactivated localization microscopy (PALM), an optical superresolution technique. Dr. Shroff is now chief of NIBIB's section on high resolution optical imaging laboratory, where he and his staff are developing new imaging tools for application in biological and clinical research.

Research Interests

• Development of new imaging tools to study fast 3D cellular processes
• Super-resolution microscopy
• Cell motility
• Single molecule fluorescence
• Physical properties of highly-bent DNA molecules

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Publications

Wu, Y., Ghitani, A., Christensen, R., Santella, A., Du, Z., Rondeau, G., Bao, Z., Colon-Ramos, D., Shroff, H. Inverted selective plane illumination microscopy (iSPIM) enables coupled cell identity lineaging and neurodevelopmental imaging in Caenorhabditis elegans. PNAS 108, 17708-17713 (2011).

York A.G., Ghitani, A., Vaziri, A., Davidson M.W., Shroff, H. Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes. Nat. Methods 4: 327-333 (2011). Click here to see the HROI Palm 3D code and software information web page.

Frost NA, Shroff H, Kong H, Betzig E, Blanpied TA. Single-molecule discrimination of discrete perisynaptic and distributed sites of actin filament assembly within dendritic spines. Neuron, 67(1):86-99, 2010.

Greenfield D, McEvoy AL, Shroff H, Crooks GE, Wingreen NS, Betzig E, Liphardt J. Self-Organization of the Escherichia coli Chemotaxis Network Imaged with Super-Resolution Light Microscopy. PLoS Biology, 7:e1000137, 2009.

Vaziri A, Tang J, Shroff H, Shank CV. Multilayer Three-Dimensional Super-resolution Imaging of Thick Samples. PNAS 105:20221-20226, 2008.

Ji N, Shroff H, Zhong H, Betzig E. Advances in the speed and resolution of light microscopy. Current Opinion in Neurobiology, 18:605-616, 2008.

Shroff H, White H, Betzig E. Photoactivation localization microscopy (PALM) of adhesion complexes. Current Protocols in Cell Biology, 4.21.1-4.21.27, 2008.

Shroff H, Galbraith, C.G., Galbraith, J.A., Betzig, E. Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics. Nat Methods, 5:417-423, 2008.

Manley S, Gillette JM, Patterson GH, Shroff H, Hess HF, Betzig E, Lippincott-Schwartz J. High-density mapping of single-molecule trajectories with photoactivated localization microscopy. Nat Methods, 5:155-157, 2008.

Shroff H, Sivak D, Siegel JJ, McEvoy A, Siu M, Spakowitz A, Geissler P, Liphardt J. Optical measurement of mechanical forces inside short DNA loops. Biophys J, 94:2179-2186, 2008.

Shroff H, Galbraith CG, Galbraith JA, White H, Gillette J, Olenych S, Davidson MW, Betzig E. Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes. PNAS, 104:20308-20313, 2007.

Pauzauskie PJ, Radenovic A, Trepagnier E, Shroff H, Yang P, Liphardt J. Optical trapping and integration of nanowire assemblies in water. Nat Materials, 5:97-101, 2006.

Shroff H, Reinhard BM, Siu M, Agarwal H, Spakowitz A, Liphardt J. Biocompatible force sensor with optical readout and dimensions of 6 nm3. Nano Lett, 5:1509-1514, 2005.

Former Lab Members

Alireza Ghitani, B.S.
Mr. Ghitani received his Bachelor of Science degree in biology from George Mason University and participated in the Postbaccalaureate IRTA program at NIBIB from 2010-2011. While in training at NIBIB, he worked with Caenorhabditis elegans using modified microscopes to look for differentiated cells and their location to analyze development.

Kelsey Temprine, B.S.
Ms. Kelsey Temprine received her Bachelor of Science degree from the University of Texas at Austin and participated in the Postbaccalaureate IRTA program at NIBIB from 2011-2012. While in training, she applied PALM to the study of bacterial sporulation and helped to develop a new form of structured illumination microscopy. She is currently a graduate student at the Memorial Sloan-Kettering Cancer Center.

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