Creating Biomedical Technologies to Improve Health

LABS AT NIBIB

Dynamics of Macromolecular Assembly Section

This is a computer generated model that shows differences in the molecular structure of the human and C. intestinalis crystallin protein which is a protein found in the vertebrate eye lens. The N terminal domain of the human protein is in yellow and the C. intestinalis is gray. Identical residues are highlighted in blue. Residues in the human protein that have higher dn over dc value compared to those at the same position in the C. intestinalis are red.

The Section of the Dynamics of Macromolecular Assembly develops biophysical methods to study protein interactions and the assembly of multi-protein complexes. Hallmarks of multi-protein complexes are multi-valent interactions and cooperativity. In the molecular machinery of cellular processes these constitute ubiquitous mechanisms for the integration and transfer of information. Therefore, our focus is on the development of approaches for multi-component systems where several different macromolecular components interact to allow association and dissociation of different co-existing complexes in different states. We are interested in the characterization of the number of assembly states, and their size, shape, and the interaction energetics. Complementary to crystallographic techniques, such solution interaction studies can provide information on the assembly principles of structurally polymorph multi-protein complexes.