Biomedical Imaging is evolving to meet new demands in biology and medicine, including the possibility to localize gene expression, to characterize cell behavior in vivo, to map out the functioning brain, or to provide personalized medicine, point-of-care delivery, and minimally invasive procedures. To address these needs, biomedical imaging methodologies are being challenged to extend resolution and contrast, to increasingly include multiple modalities, operate in various clinical and research settings, and focus on a variety of temporal and spatial scales, from molecular to whole-body imaging, from nano to macro. The eighth IEEE International Symposium on Biomedical Imaging (ISBI’11) will be held 29 March- 2 April 2011, at the Hyatt Regency McCormick Place in Chicago Illinois, USA. ISBI is a joint initiative of the IEEE Signal Processing Society (SPS) and the IEEE Engineering in Medicine and Biology Society (EMBS). Previous meetings have played a leading role in facilitating interaction between researchers in medical and biological imaging. The 2011 meeting will continue the tradition of fostering cross-fertilization between different imaging communities and contributing to an integrative imaging approach across all scales of observation.