Field: Fluorescence microscopy
Position description: The Section on Biophotonics within the National Institute of Biomedical Imaging and Bioengineering (NIBIB) of the National Institutes of Health (NIH) is seeking a post-doctoral fellow with experience in fluorescence microscopy. The lab develops probes and techniques for use in diffraction-limited and super-resolution fluorescence imaging of cells and tissues. The team improves upon and exploits existing developments in fluorescent proteins, instrumentation/methodology, and image analysis to better serve the cell and developmental biology communities. Current approaches take advantage of the photophysical behavior of fluorescent probes to improve the information content of the data being collected. For example, recent and ongoing studies take advantage of the behaviors of photoswitchable fluorescent proteins as they transition between their “off” and “on” states to monitor Förster resonance energy transfer (FRET) in living cells1,2. Ongoing multiplexing developments of these approaches are designed to better facilitate multiple FRET readouts within the same cell. The team has exciting collaborative efforts underway to image protein-protein interactions at key points in the secretory pathway in addition to efforts to improve upon and utilize fluorescent protein-based biosensors using these photoswitching approaches. A successful candidate will possess the creativity, motivation, and experience to help drive these developments.
1. Ojha, N., Rainey, K.R., and Patterson G.H. Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association. Nat Commun. 2020 Jan 7;11(1):21. PMID: 31911590
2. Rainey, K.R. and Patterson G.H. Photoswitching FRET to monitor protein-protein interactions. Proc Natl Acad Sci U S A. 2019. 116(3):864-873. PMID: 30598438
Qualifications: The applicant should have a Ph.D. in cell biology, biophysics, biochemistry, bioengineering, or related discipline. A successful candidate will have experience with one or more modes of fluorescence imaging, fluorescence image analyses, standard molecular biology techniques, and common cell culture techniques. Experience with programming is not required but is considered beneficial.
To Apply: Candidates should send inquiries to George H. Patterson (firstname.lastname@example.org). Please include an updated CV, the names and email addresses of three references, and the date of earliest availability.
Position includes salary and benefits commensurate with NIH guidelines for post-doctoral fellows.
Location: Building 13, NIH main campus, Bethesda, MD
The National Institutes of Health is an equal opportunity employer. The NIH is dedicated to building a diverse community in its training and employment programs.