Section on Mechanobiology

The focus of the Section on Mechanobiology is the development and use of advanced Atomic Force Microscopy (AFM) technologies for cellular and tissue mechanics investigation. We combine and apply diverse multidisciplinary knowledge to investigate the most pressing mechanobiology questions, including high spatio-temporal resolution AFM and confocal fluorescent microscopes, image analysis, and mathematical modeling.

The Section on Mechanobiology seeks to understand several important biological processes by applying physics and engineering principles, particularly: the molecular-mechanical regulation of the actomyosin cortex of melanoma cells; the solid tumor microenvironment for deciphering self-organization in cancer biology; and the anisotropic mechanical properties of developmental and mature inner ear sensory and non-sensory epithelial tissues using a novel noncontact AFM approach.

Additionally, the laboratory develops new AFM methodologies to study fast multiparametric and multidimensional cellular and tissue processes, and advances the state-of-the-art AFM imaging methods for high spatio-temporal and quantitative nanomechanical mapping.

Alex Cartagena Rivera looking through a microscope
Alex Cartagena-Rivera, PhD. (lab PI) 
Photo: C Chang for NIBIB

 

Molecular-Mechanical Regulation in Cancer Biology
The aim of this project is to investigate the molecular-mechanical regulation of the actomyosin cortex of melanoma cells and the solid tumor microenvironment for deciphering self-organization in cancer biology.

Metastatic melanoma is the deadliest form of skin cancer, expected to kill ~7,230 people in the United States in 2019 alone (estimated by cancer.org). Different cells and mediators in the tumor microenvironment play important roles in the progression of cancer. To successfully metastasize to a distal site, melanoma cells use a variety of migration modalities that critically depend on spatial tumor microenvironment changes and changes in actomyosin cytoskeleton organization. Thus, the ability of tumor cells to adapt is a critical driver of the upregulated cancer cell motility and invasiveness which leads to tumor progression and metastasis. This cell adaption process has been shown to be highly mechanosensitive, thus mechanical properties may play an important role in fine regulating tumor progression and aggression.

We have recently developed multiple advanced atomic force microscopy (AFM) methodologies to determine the nanoscale and global mechanical properties of cells1,2. AFM is a versatile biomedical technique in which a sample is probed with a microcantilever with or without an indenter to non-invasively measure the physical material properties of complex biological systems in a physiologically relevant environment. A major advantage of this experimental approach is that it does not require any chemical manipulation (neither labeling or fixation).

First, we used a recently developed quasi-static AFM force spectroscopy method that employs tipless cantilevers to determine global physical relevant mechanical properties including actomyosin tension, elastic Young’s modulus, viscosity, and intracellular pressure2. We sought to investigate the contribution of formin activity in actomyosin cortex self-organization and mechanical regulation in melanoma cells.

Second, we implemented high spatial and temporal resolution PeakForce Tapping AFM and JPK QI modalities to map the nanoscale mechanical properties of the microenvironment of melanoma solid tumors. This enabled us to examine the changes in the mechanical properties' heterogeneity and structural self-organization that resulted in metastatic progression.

1Efremov, Y. M.*, Cartagena-Rivera, A. X.*, Athamneh, A. I. M., Suter, D. M. & Raman, A. Mapping heterogeneity of cellular mechanics by multi-harmonic atomic force microscopy. Nat. Proct. 13, 2200-2216 (2018) *Equal contribution
2Cartagena-Rivera, A. X., Logue, J. S., Waterman, C. M. & Chadwick, R. S. Actomyosin cortical mechanical properties in nonadherent cells determined by atomic force microscopy. Biophys. J. 110, 2528-2539 (2016)

Mechanical Properties of Inner Ear Tissues
The goal of this project is to determine the anisotropic mechanical properties of developmental and mature inner ear sensory and non-sensory epithelial tissues using a novel noncontact AFM approach.

It is estimated that more than 38 million people in the United States suffer some form of hearing trouble. Additionally, in the U.S., for every 1,000 newborns, two to three are born with detectable levels of hearing loss3. Hearing loss often results from dysfunction of sensory and non-sensory epithelia in the cochlea. Sound stimuli deflect stereociliary bundles that project from the apical surface of inner and outer hair cells and open mechanotransduction channels. The apical surface of interconnected cells (hair cells and supporting cells) in the sensory epithelia need to be in mechanical equilibrium for normal hearing. Perturbations to this equilibrium cause modification to the force transmission causing a defect in sound detection and transmission. Therefore, the molecular-mechanical regulation of forces within sensory and non-sensory epithelia needs to be carefully investigated.

Formerly, we developed a noninvasive AFM method that uses acoustic frequency modulation curves acquired using cantilevers with an attached microsphere to determine the supracellular apical surface tension, effective viscosity, and intercellular adhesive forces in polarized epitheliums4,5. More recently, we developed an advanced AFM methodology that builds upon our previously described noncontact frequency modulation AFM capable of measuring the strong anisotropy of the mechanical properties in tissues. We are currently using this new method to explore the anisotropic mechanical properties of developmental and mature inner ear sensory and non-sensory epithelia.

3NIDCD Health statistics web page 
4Cartagena-Rivera, A. X., Van Itallie, C. M., Anderson, J. M. & Chadwick, R. S. Apical surface supracellular mechanical properties in polarized epithelium using noninvasive acoustic force spectroscopy. Nat. Commun. 8, 1030 (2017)
5Cartagena-Rivera, A. X.*, Le Gal, S., Richards, K., Verpy, E. & Chadwick, R. S.* Cochlear outer hair cell horizontal top connectors mediate mature stereocilia bundle mechanics. Sci. Adv. 5, eaat9934 (2019) *Co-corresponding authors

 

The Cartagena-Rivera lab currently houses:

  • A Bruker BioScope Catalyst Atomic Force Microscope (AFM) combined with an inverted optical microscope Zeiss confocal LSM 510 META.
  • A Polytec single-point laser Doppler vibrometer for sub-nanometer measurement of oscillations.
Katsuno TBelyantseva IACartagena-Rivera AXOhta KCrump SMPetralia RSOno KTona RImtiaz ARehman AKiyonari HKaneko MWang YXAbe TIkeya MFenollar-Ferrer CRiordan GPWilson EAFitzgerald TSSegawa KOmori KIto JFrolenkov GIFriedman TBKitajiri SI
JCI Insight
2019 Jun 20

Cartagena-Rivera AXLe Gal SRichards KVerpy EChadwick RS
Sci Adv
2019 Feb


Efremov YMCartagena-Rivera AXAthamneh AIMSuter DMRaman A
Nat Protoc
2018 Oct

Logue JSCartagena-Rivera AXChadwick RS
Oncogene
2018 Apr

Van Itallie CMTietgens AJAponte AGucek MCartagena-Rivera AXChadwick RSAnderson JM
J. Cell. Sci.
2018 Feb 02


Cartagena-Rivera AXLogue JSWaterman CMChadwick RS
Biophys. J.
2016 Jun 07

Athamneh AICartagena-Rivera AXRaman ASuter DM
Biophys. J.
2015 Oct 06

Logue JSCartagena-Rivera AXBaird MADavidson MWChadwick RSWaterman CM
Elife
2015 Jul 11