Advanced Imaging and Microscopy (AIM) Resource

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The Advanced Imaging & Microscopy Resource is a trans-NIH shared resource that houses, operates, disseminates, and improves non-commercial, prototype optical imaging systems developed at the NIH. The facilities at AIM are available for use by the entire NIH intramural research community.

While we welcome users with any size imaging project, AIM specializes in large, year long (or longer), collaborative research efforts with NIH labs – providing sample preparation, microscope operation and development, and data processing/analysis as needed. We take care of the imaging and processing so that you can focus on the biology.

Microscope Description Spatial Resolution Speed
(frames/s)
Photodose Sample

Instant structured illumination microscope (ISIM)

Fast super-resolution

140nm lateral
350nm axial

100

High

Live or fixed. Small, thin samples like cells, organoids.

Dual-view Inverted Selective Plane Illumination Microscope (DISPIM)

Fast, gentle, high resolution lightsheet for live samples

330nm isotropic

100

Low

Live or fixed. Cells, up to medium size, transparent samples like organoids, C. elegans, zebrafish.

Cleared Tissue DISPIM

Fast multi-view lightsheet for large cleared tissue samples

500nm isotropic

100

Low

Fixed and cleared. Up to 5mm thick slabs of tissue, up to 2cm x 3cm lateral size.

Single Molecule TIRF/Inclined Illumination Microscope

Variable inclination illumination single molecule imaging. Including STORM. 

20 nm lateral
50-60 nm axial

100

High

Live or fixed. Small, thin samples like cells.

Interferometric Photoactivated Localization Microscope (IPALM)

Localization microscope with unprecedented lateral and axial resolution.

20 nm lateral
10nm axial

Not suitable for live cells

High

Fixed and specially mounted. Small and thin samples like cells, bacteria.

This table describes the spatial resolution, speed, and sample suitability of the five microscopes currently being operated by the Advanced Imaging and Microscopy (AIM) Resource.

 

 

Additional Lab Resource Information: 

Strepay DOlszewski RTNixon SKorrapati SAdadey SGriffith AJSu YLiu JVishwasrao HGu SSaunders TRoux IHoa M
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2024 Feb 06

Lokwani RJosyula ANgo TBDeStefano SFertil DFaust MAdusei KMBhuiyan MLin AKarkanitsa MMaclean EFathi PSu YLiu JVishwasrao HDSadtler K
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Sadhu RKHernandez-Padilla CEisenbach YEPenič SZhang LVishwasrao HDBehkam BKonstantopoulos KShroff HIglič APeles ENain ASGov NS
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Nguyen AZhao HMyagmarsuren DSrinivasan SWu DChen JPiszczek GSchuck P
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Strepay DOlszewski RTNixon SKorrapati SAdadey SGriffith AJSu YLiu JVishwasrao HGu SSaunders TRoux IHoa M
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Sadhu RKHernandez-Padilla CEisenbach YEPenič SZhang LVishwasrao HDBehkam BKonstantopoulos KShroff HIglič APeles ENain ASGov NS
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Sun YBaechler SAZhang XKumar SFactor VMArakawa YChau CHOkamoto KParikh AWalker BSu YPChen JTing THuang SNBeck EItkin ZMcKnight CXie CRoper NNijhawan DFigg WDMeltzer PSYang JCThomas CJPommier Y
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Zhao HWu DHassan SANguyen AChen JPiszczek GSchuck P
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Ngo TBDeStefano SLiu JSu YShroff HVishwasrao HDSadtler K
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Li XWu YSu YRey-Suarez IMatthaeus CUpdegrove TBWei ZZhang LSasaki HLi YGuo MGiannini JPVishwasrao HDChen JLee SJShao LLiu HRamamurthi KSTaraska JWUpadhyaya ALa Riviere PShroff H
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Nature
2021 Dec

Sun YChen JHuang SNSu YPWang WAgama KSaha SJenkins LMPascal JMPommier Y
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Chen JSasaki HLai HSu YLiu JWu YZhovmer ACombs CARey-Suarez IChang HYHuang CCLi XGuo MNizambad SUpadhyaya ALee SJLucas LAGShroff H
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Han XSu YWhite HO'Neill KMMorgan NYChristensen RPotarazu DVishwasrao HDXu SSun YHuang SYMoyle MWDai QPommier YGiniger EAlbrecht DRProbst RShroff H
Lab Chip
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Miyoshi TZhang QMiyake TWatanabe SOhnishi HChen JVishwasrao HDChakraborty OBelyantseva IAPerrin BJShroff HFriedman TBOmori KWatanabe N
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Guo MLi YSu YLambert TNogare DDMoyle MWDuncan LHIkegami RSantella ARey-Suarez IGreen DBeiriger AChen JVishwasrao HGanesan SPrince VWaters JCAnnunziata CMHafner MMohler WAChitnis ABUpadhyaya AUsdin TBBao ZColón-Ramos DLa Riviere PLiu HWu YShroff H
Nat Biotechnol
2020 Nov

Chen JLiu YWu BNikolaitchik OAMohan PRChen JPathak VKHu WS
Proc Natl Acad Sci U S A
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Gryder BEPomella SSayers CWu XSSong YChiarella AMBagchi SChou HCSinniah RSWalton AWen XRota RHathaway NAZhao KChen JVakoc CRShern JFStanton BZKhan J
Nat Genet
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Peluso EAUpdegrove TBChen JShroff HRamamurthi KS
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Liu YGong CSDai YYang ZYu GLiu YZhang MLin LTang WZhou ZZhu GChen JJacobson OKiesewetter DOWang ZChen X
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Mo TTDai HDu HZhang RYChai KPAn YChen JJWang JKChen ZJChen CZJiang XJTang RWang LPTan QTang PMiao XYMeng PZhang LBCheng SQPeng BTu BJHan TLXia YYBaker PN
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2018 Jun


Zhu GLynn GMJacobson OChen KLiu YZhang HMa YZhang FTian RNi QCheng SWang ZLu NYung BCWang ZLang LFu XJin AWeiss IDVishwasrao HNiu GShroff HKlinman DMSeder RAChen X
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Qian ZZhurkin VBAdhya S
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