Harshad Vishwasrao

Harshad Vishwasrao
Staff Scientist

BG 13 RM G800 13 South Dr. Bethesda MD 20814


Dr. Harshad Vishwasrao received his BA in physics and mathematics in 1997 from the University of Pennsylvania. He then did his PhD (2005) with Watt Webb at Cornell University, where he used two-photon microscopy and ultrafast time resolved anisotropy to study the conformational dynamics of the intrinsic fluorophore NADH in the brain as a function of metabolic activity. Taking a detour into neuroscience, he took positions as first a post-doctoral fellow and then a research scientist with Eric Kandel at Columbia University. His interest in the plasticity induced remodeling of the synapse lead him to develop two fluorescence microscopy techniques. The first was a homo-FRET/polarization based approach to quantitatively image the actin polymer fraction in live cells. The second was a method of iterative multiplex imaging using selectively chemically cleavable immunoprobes. 

Dr. Vishwasrao joined the NIH in 2016 as the co-Operating Director of the Advanced Imaging and Microscopy Resource.


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Nat Biotechnol
2020 Nov

Guo MChandris PGiannini JPTrexler AJFischer RChen JVishwasrao HDRey-Suarez IWu YWu XWaterman CMPatterson GHUpadhyaya ATaraska JWShroff H
Nat Methods
2018 Jun

Zhu GLynn GMJacobson OChen KLiu YZhang HMa YZhang FTian RNi QCheng SWang ZLu NYung BCWang ZLang LFu XJin AWeiss IDVishwasrao HNiu GShroff HKlinman DMSeder RAChen X
Nat Commun
2017 Dec 05

Zhu GMei LVishwasrao HDJacobson OWang ZLiu YYung BCFu XJin ANiu GWang QZhang FShroff HChen X
Nat Commun
2017 Nov 14

Wu YKumar ASmith CArdiel EChandris PChristensen RRey-Suarez IGuo MVishwasrao HDChen JTang JUpadhyaya ALa Riviere PJShroff H
Nat Commun
2017 Nov 13

Qian ZZhurkin VBAdhya S
Proc Natl Acad Sci U S A
2017 Nov 14

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Mol Cell
2017 Aug 17

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J Cell Sci
2017 Sep 01