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Jiji Chen

Jiji Chen
Jiji
Chen
Ph.D.
Staff Scientist
Advanced Imaging and Microscopy (AIM) Resource
Clickable Email icon
jiji.chen@nih.gov
Biography

Dr. Chen received his PhD in 2011 from Purdue University under Joseph Irudayaraj. His doctoral work utilized single molecule spectroscopy and time resolved techniques to study epigenetic modifications in single cells. He then joined the Transcription Imaging Consortium at the HHMI Janelia Research Campus to do his post-doctoral fellowship with Maxime Dahan and Robert Tjian. At Janelia, Dr. Chen co-developed the multifocus microscope – a widefield fluorescence microscope capable of imaging multiple focal planes simultaneously. He then combined multifocus microscopy with single molecule imaging to quantitatively track the interactions of transcription factors at endogenous binding elements in 3D in live cells.

Dr. Chen joined the NIH in 2016 as the Co-Operating Director of the Advanced Imaging and Microscopy Resource.

Publications
The HIV-1 Viral Protease Is Activated during Assembly and Budding Prior to Particle Release.
Tabler COWegman SJChen JShroff HAlhusaini NTilton JC
J Virol
2022 May 11

Plasticity in structure and assembly of SARS-CoV-2 nucleocapsid protein.
Zhao HNguyen AWu DLi YHassan SAChen JShroff HPiszczek GSchuck P
bioRxiv
2022 Feb 09

Multiview confocal super-resolution microscopy.
Wu YHan XSu YGlidewell MDaniels JSLiu JSengupta TRey-Suarez IFischer RPatel ACombs CSun JWu XChristensen RSmith CBao LSun YDuncan LHChen JPommier YShi YBMurphy ERoy SUpadhyaya AColón-Ramos DLa Riviere PShroff H
Nature
2021 Dec

PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation.
Sun YChen JHuang SNSu YPWang WAgama KSaha SJenkins LMPascal JMPommier Y
Nat Commun
2021 Aug 18

Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes.
Chen JSasaki HLai HSu YLiu JWu YZhovmer ACombs CARey-Suarez IChang HYHuang CCLi XGuo MNizambad SUpadhyaya ALee SJLucas LAGShroff H
Nat Methods
2021 Jun

Semi-automated single-molecule microscopy screening of fast-dissociating specific antibodies directly from hybridoma cultures.
Miyoshi TZhang QMiyake TWatanabe SOhnishi HChen JVishwasrao HDChakraborty OBelyantseva IAPerrin BJShroff HFriedman TBOmori KWatanabe N
Cell Rep
2021 Feb 02

Quantum Dot-Conjugated SARS-CoV-2 Spike Pseudo-Virions Enable Tracking of Angiotensin Converting Enzyme 2 Binding and Endocytosis.
Gorshkov KSusumu KChen JXu MPradhan MZhu WHu XBreger JCWolak MOh E
ACS Nano
2020 Sep 22

Rapid image deconvolution and multiview fusion for optical microscopy.
Guo MLi YSu YLambert TNogare DDMoyle MWDuncan LHIkegami RSantella ARey-Suarez IGreen DBeiriger AChen JVishwasrao HGanesan SPrince VWaters JCAnnunziata CMHafner MMohler WAChitnis ABUpadhyaya AUsdin TBBao ZColón-Ramos DLa Riviere PLiu HWu YShroff H
Nat Biotechnol
2020 Nov

Visualizing the translation and packaging of HIV-1 full-length RNA.
Chen JLiu YWu BNikolaitchik OAMohan PRChen JPathak VKHu WS
Proc Natl Acad Sci U S A
2020 Mar 17

Histone hyperacetylation disrupts core gene regulatory architecture in rhabdomyosarcoma.
Gryder BEPomella SSayers CWu XSSong YChiarella AMBagchi SChou HCSinniah RSWalton AWen XRota RHathaway NAZhao KChen JVakoc CRShern JFStanton BZKhan J
Nat Genet
2019 Dec

A 2-dimensional ratchet model describes assembly initiation of a specialized bacterial cell surface.
Peluso EAUpdegrove TBChen JShroff HRamamurthi KS
Proc Natl Acad Sci U S A
2019 Oct 22

In situ polymerization on nanoscale metal-organic frameworks for enhanced physiological stability and stimulus-responsive intracellular drug delivery.
Liu YGong CSDai YYang ZYu GLiu YZhang MLin LTang WZhou ZZhu GChen JJacobson OKiesewetter DOWang ZChen X
Biomaterials
2019 Oct

Single-shot super-resolution total internal reflection fluorescence microscopy.
Guo MChandris PGiannini JPTrexler AJFischer RChen JVishwasrao HDRey-Suarez IWu YWu XWaterman CMPatterson GHUpadhyaya ATaraska JWShroff H
Nat Methods
2018 Jun

A RAS-CaMKKβ-AMPKα2 pathway promotes senescence by licensing post-translational activation of C/EBPβ through a novel 3'UTR mechanism.
Basu SKGonit MSalotti JChen JBhat AGorospe MViollet BClaffey KPJohnson PF
Oncogene
2018 Jun

Albumin/vaccine nanocomplexes that assemble in vivo for combination cancer immunotherapy.
Zhu GLynn GMJacobson OChen KLiu YZhang HMa YZhang FTian RNi QCheng SWang ZLu NYung BCWang ZLang LFu XJin AWeiss IDVishwasrao HNiu GShroff HKlinman DMSeder RAChen X
Nat Commun
2017 Dec 05

Intertwining DNA-RNA nanocapsules loaded with tumor neoantigens as synergistic nanovaccines for cancer immunotherapy.
Zhu GMei LVishwasrao HDJacobson OWang ZLiu YYung BCFu XJin ANiu GWang QZhang FShroff HChen X
Nat Commun
2017 Nov 14

Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy.
Wu YKumar ASmith CArdiel EChandris PChristensen RRey-Suarez IGuo MVishwasrao HDChen JTang JUpadhyaya ALa Riviere PJShroff H
Nat Commun
2017 Nov 13

DNA-RNA interactions are critical for chromosome condensation in .
Qian ZZhurkin VBAdhya S
Proc Natl Acad Sci U S A
2017 Nov 14

Myc Regulates Chromatin Decompaction and Nuclear Architecture during B Cell Activation.
Kieffer-Kwon KRNimura KRao SSPXu JJung SPekowska ADose MStevens EMathe EDong PHuang SCRicci MABaranello LZheng YTomassoni Ardori FResch WStavreva DNelson SMcAndrew MCasellas AFinn EGregory CSt Hilaire BGJohnson SMDubois WCosma MPBatchelor ELevens DPhair RDMisteli TTessarollo LHager GLakadamyali MLiu ZFloer MShroff HAiden ELCasellas R
Mol Cell
2017 Aug 17

Probing the target search of DNA-binding proteins in mammalian cells using TetR as model searcher.
Normanno DBoudarène LDugast-Darzacq CChen JRichter CProux FBénichou OVoituriez RDarzacq XDahan M
Nat Commun
2015 Jul 07

A general method to improve fluorophores for live-cell and single-molecule microscopy.
Grimm JBEnglish BPChen JSlaughter JPZhang ZRevyakin APatel RMacklin JJNormanno DSinger RHLionnet TLavis LD
Nat Methods
2015 Mar

Whole-cell, multicolor superresolution imaging using volumetric multifocus microscopy.
Hajj BWisniewski JEl Beheiry MChen JRevyakin AWu CDahan M
Proc Natl Acad Sci U S A
2014 Dec 09

Imaging the fate of histone Cse4 reveals de novo replacement in S phase and subsequent stable residence at centromeres.
Wisniewski JHajj BChen JMizuguchi GXiao HWei DDahan MWu C
Elife
2014 May 20

Single-molecule dynamics of enhanceosome assembly in embryonic stem cells.
Chen JZhang ZLi LChen BCRevyakin AHajj BLegant WDahan MLionnet TBetzig ETjian RLiu Z
Cell
2014 Mar 13