Jiji Chen

Jiji

Chen

Ph.D.

Staff Scientist

Advanced Imaging and Microscopy (AIM) Resource

BG 13 RM G800 13 South Dr. Bethesda MD 20892

jiji.chen@nih.gov

Biography

Dr. Chen received his PhD in 2011 from Purdue University under Joseph Irudayaraj. His doctoral work utilized single molecule spectroscopy and time resolved techniques to study epigenetic modifications in single cells. He then joined the Transcription Imaging Consortium at the HHMI Janelia Research Campus to do his post-doctoral fellowship with Maxime Dahan and Robert Tjian. At Janelia, Dr. Chen co-developed the multifocus microscope – a widefield fluorescence microscope capable of imaging multiple focal planes simultaneously. He then combined multifocus microscopy with single molecule imaging to quantitatively track the interactions of transcription factors at endogenous binding elements in 3D in live cells.

Dr. Chen joined the NIH in 2016 as the Co-Operating Director of the Advanced Imaging and Microscopy Resource.

Publications

Histone hyperacetylation disrupts core gene regulatory architecture in rhabdomyosarcoma.

Gryder BEPomella SSayers CWu XSSong YChiarella AMBagchi SChou HCSinniah RSWalton AWen XRota RHathaway NAZhao KChen JVakoc CRShern JFStanton BZKhan J

Nat. Genet.

2019 Dec

A 2-dimensional ratchet model describes assembly initiation of a specialized bacterial cell surface.

Peluso EAUpdegrove TBChen JShroff HRamamurthi KS

Proc. Natl. Acad. Sci. U.S.A.

2019 Oct 22

In situ polymerization on nanoscale metal-organic frameworks for enhanced physiological stability and stimulus-responsive intracellular drug delivery.

Liu YGong CSDai YYang ZYu GLiu YZhang MLin LTang WZhou ZZhu GChen JJacobson OKiesewetter DOWang ZChen X

Biomaterials

2019 Oct

Single-shot super-resolution total internal reflection fluorescence microscopy.

Guo MChandris PGiannini JPTrexler AJFischer RChen JVishwasrao HDRey-Suarez IWu YWu XWaterman CMPatterson GHUpadhyaya ATaraska JWShroff H

Nat. Methods

2018 Jun

A RAS-CaMKKβ-AMPKα2 pathway promotes senescence by licensing post-translational activation of C/EBPβ through a novel 3'UTR mechanism.

Basu SKGonit MSalotti JChen JBhat AGorospe MViollet BClaffey KPJohnson PF

Oncogene

2018 Jun

Albumin/vaccine nanocomplexes that assemble in vivo for combination cancer immunotherapy.

Zhu GLynn GMJacobson OChen KLiu YZhang HMa YZhang FTian RNi QCheng SWang ZLu NYung BCWang ZLang LFu XJin AWeiss IDVishwasrao HNiu GShroff HKlinman DMSeder RAChen X

Nat Commun

2017 Dec 05

Intertwining DNA-RNA nanocapsules loaded with tumor neoantigens as synergistic nanovaccines for cancer immunotherapy.

Zhu GMei LVishwasrao HDJacobson OWang ZLiu YYung BCFu XJin ANiu GWang QZhang FShroff HChen X

Nat Commun

2017 Nov 14

Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy.

Wu YKumar ASmith CArdiel EChandris PChristensen RRey-Suarez IGuo MVishwasrao HDChen JTang JUpadhyaya ALa Riviere PJShroff H

Nat Commun

2017 Nov 13

DNA-RNA interactions are critical for chromosome condensation in .

Qian ZZhurkin VBAdhya S

Proc. Natl. Acad. Sci. U.S.A.

2017 Nov 14

Myc Regulates Chromatin Decompaction and Nuclear Architecture during B Cell Activation.

Kieffer-Kwon KRNimura KRao SSPXu JJung SPekowska ADose MStevens EMathe EDong PHuang SCRicci MABaranello LZheng YTomassoni Ardori FResch WStavreva DNelson SMcAndrew MCasellas AFinn EGregory CSt Hilaire BGJohnson SMDubois WCosma MPBatchelor ELevens DPhair RDMisteli TTessarollo LHager GLakadamyali MLiu ZFloer MShroff HAiden ELCasellas R

Mol. Cell

2017 Aug 17

Probing the target search of DNA-binding proteins in mammalian cells using TetR as model searcher.

Normanno DBoudarène LDugast-Darzacq CChen JRichter CProux FBénichou OVoituriez RDarzacq XDahan M

Nat Commun

2015 Jul 07

A general method to improve fluorophores for live-cell and single-molecule microscopy.

Grimm JBEnglish BPChen JSlaughter JPZhang ZRevyakin APatel RMacklin JJNormanno DSinger RHLionnet TLavis LD

Nat. Methods

2015 Mar

Whole-cell, multicolor superresolution imaging using volumetric multifocus microscopy.

Hajj BWisniewski JEl Beheiry MChen JRevyakin AWu CDahan M