Harshad Vishwasrao
, Ph.D.
Acting Director
| Microscope | Description | Spatial Resolution | Speed (frames/s) |
Photodose | Sample |
|---|---|---|---|---|---|
| Instant structured illumination microscope (ISIM) | Fast super-resolution | 140nm lateral 350nm axial |
100 | High | Live or fixed. Small, thin samples like cells, organoids. |
| Dual-view Inverted Selective Plane Illumination Microscope (DISPIM) | Fast, gentle, high resolution lightsheet for live samples | 330nm isotropic | 100 | Low | Live or fixed. Cells, up to medium size, transparent samples like organoids, C. elegans, zebrafish. |
| Cleared Tissue DISPIM | Fast multi-view lightsheet for large cleared tissue samples | 500nm isotropic | 100 | Low | Fixed and cleared. Up to 5mm thick slabs of tissue, up to 2cm x 3cm lateral size. |
| Single Molecule TIRF/Inclined Illumination Microscope | Variable inclination illumination single molecule imaging. Including STORM. | 20 nm lateral 50-60 nm axial |
100 | High | Live or fixed. Small, thin samples like cells. |
| Interferometric Photoactivated Localization Microscope (IPALM) | Localization microscope with unprecedented lateral and axial resolution. | 20 nm lateral 10nm axial |
Not suitable for live cells | High | Fixed and specially mounted. Small and thin samples like cells, bacteria. |
This table describes the spatial resolution, speed, and sample suitability of the five microscopes currently being operated by the Advanced Imaging and Microscopy (AIM) Resource.
Additional Lab Resource Information:
- Advanced Image Processing (NEW)
- Dualview Inverted Selective Plane Illumination Microscope (DISPIM)
- Instant Structured Illumination Microscope (ISIM)/ Total Internal Reflection Fluorescence SIM (TIRF-SIM)
- Interferometric Photoactivated Localization Microscope (IPALM)
- High throughput single molecule imaging microscope
- Single molecule TIRF/Inclined Illumination Microscope