Single molecule TIRF/Inclined illumination Microscope

This single molecule detection microscope is based on widefield illumination with the flexibility of tuning between TIRF and inclined illumination for different applications - enabling localization based imaging techniques including single molecule tracking, PALM and dSTORM. The TIRF illumination enables the monitoring of single molecule interactions on the glass coverslips or the imaging of biomolecules in a very thin layer in live cells (mostly on the basal plasma membrane). With the live cell environment system, it also allows single molecule tracking in live cells.

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Image showing instrumentation of single molecule imaging microscopy
Figure 1. Instrumentation of single molecule imaging microscopy


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