The ISIM is a fluorescence microscope that combines rapid image acquisition (100Hz) with a resolution roughly double that of a confocal microscope in all three dimensions. Unlike other structured illumination microscopes, the resolution is enhanced via hardware in a single frame acquisition rather than computationally processing multiple images, effectively making this an ‘instantaneous’ structured illumination microscope. Recently, the Shroff Lab has extended the capability of this microscope to rapid, super-resolution TIRF.
Spatial resolution: 140nm lateral, 350nm axial
Temporal resolution: Framerate up to 100Hz
Excitation wavelengths: 488nm, 561nm and 639nm
1. Instant super-resolution imaging in live cells and embryos via analog image processing. Nat Methods. 2013 Nov;10(11):1122-6.
2. Single-shot super-resolution total internal reflection fluorescence microscopy. Nat Methods. 2018 Jun;15(6):425-428.