High throughput single molecule imaging microscope

The high throughput single molecule microscope was purpose designed to image large numbers of cell samples in a multi-well format. This microscope can routinely image up to 8 coverslips and 10,000+ cells per acquisition. An included analysis pipeline automatically segments images to identify nuclei, cell bodies, and puncta (RNAs, receptors, etc.), and generates statistics on these puncta (number/cell, co-expression, co-localization, etc.). This microscope is currently located in the Larson Lab and will move to AIM once the facility is operational.

Image Gallery

High throughput single molecule FISH
Figure 1. High throughput single molecule FISH

 

Example of pipeline for single-molecule RNA FISH Analysis
Figure 2. Pipeline for single-molecule RNA FISH Analysis