![Photo of Harsh Vishwasrao](/sites/default/files/staff/Harsh%20Vishwasrao%20Staff%20Photo.jpg)
Harshad Vishwasrao, Ph.D.
The high throughput single molecule microscope was designed to image large numbers of cell samples in a multi-well format. This microscope can routinely image up to 8 coverslips and 10,000+ cells per acquisition. An included analysis pipeline automatically segments images to identify nuclei, cell bodies, and puncta (RNAs, receptors, etc.) and generates statistics on these puncta (number/cell, co-expression, co-localization, etc.). This microscope is currently located in the Larson Lab and will move to AIM once the facility is operational.
Image Gallery
![High throughput single molecule FISH](/sites/default/files/inline-images/high-throughput-single-molecule.jpg)
![Example of pipeline for single-molecule RNA FISH Analysis](/sites/default/files/inline-images/pipeline_for_single-molecule_rna_fish_analysis_0.jpg)