George Harold Patterson

George Harold Patterson

BG 13 RM 3E33 13 South Dr Bethesda MD 20814


George Patterson received a bachelor's degree for study in biology and research on the reproductive cycle of a freshwater mussel, Cyclonais tuberculata, from the University of North Alabama (Florence, Alabama) in 1992.  In 1993, he was accepted into the Interdisciplinary Graduate Program within the Vanderbilt School of Medicine, Vanderbilt University, Nashville, Tennessee. He joined the lab of David Piston in the Department of Molecular Physiology in 1994 and was awarded a PhD in 1999 for his study of glucose metabolism in pancreatic islets of Langerhans and quantitative fluorescence imaging using fluorescent proteins.  He accepted a post-doctoral position in the lab of Jennifer Lippincott-Schwartz in the Cell Biology and Metabolism Branch (National Institute of Child Health and Human Development, National Institutes of Health) in Bethesda, Maryland.  During his post-doctoral training, he developed reagents and techniques for fluorescence imaging in the study of secretory protein trafficking.  After 4 years, he accepted a staff scientist position at the NIH, where he continued work in protein trafficking, collaborative efforts with Eric Betzig and Harald Hess (Howard Hughes Medical Institute, Janelia Farm) in development of new fluorescence imaging techniques, and collaborative work with Vladislav Verkhusha (Albert Einstein School of Medicine, Bronx, New York) in development of new fluorescence imaging reagents.  He accepted a position in the National Institute of Biomedical Imaging and Bioengineering at the NIH in October 2009 where he continues work on fluorescent protein development and super-resolution imaging techniques for use in cell biology applications.

Research Interests

The NIBIB Section on Biophotonics develops probes and techniques for use in diffraction limited and sub-diffraction limited fluorescence imaging of cells and tissues. These include Photo-activated localization microscopy (PALM) and other molecular localization techniques which are based on imaging of single fluorescent molecules and Multi-focal Structured Illumination Microscopy (MSIM) which is based on exciting fluorophores with an array of diffraction-limited spots followed processing to achieve an approximately 2-fold improvement in resolution.  Major emphasis is placed on developing new and improving existing genetically encoded fluorescent proteins for use as markers and sensors.  This approach utilizes structure based mutagenesis followed by spectroscopy and imaging characterization of the variants.  Methods and technologies include multi-photon excitation microscopy, confocal microscopy, total internal reflection fluorescence (TIRF) microscopy, and widefield microscopy, single molecule imaging, fluorescence spectroscopy, and protein engineering.

Ingaramo MYork AGWawrzusin PMilberg OHong AWeigert RShroff HPatterson GH
Proc. Natl. Acad. Sci. U.S.A.
2014 Apr 08

Ingaramo MYork AGHoogendoorn EPostma MShroff HPatterson GH
2014 Mar 17

Yang JSingh VCha BChen TESarker RMurtazina RJin SZachos NCPatterson GHTse CMKovbasnjuk OLi XDonowitz M
J. Biol. Chem.
2013 Jun 07

Zhao HBerger AJBrown PHKumar JBalbo AMay CACasillas ELaue TMPatterson GHMayer MLSchuck P
J. Gen. Physiol.
2012 May

Datta SRPatterson GH
Curr. Opin. Neurobiol.
2012 Feb

Subach OMPatterson GHTing LMWang YCondeelis JSVerkhusha VV
Nat. Methods
2011 Jul 31

Subach FVPatterson GHRenz MLippincott-Schwartz JVerkhusha VV
J. Am. Chem. Soc.
2010 May 12

Patterson GH
Semin. Cell Dev. Biol.
2009 Oct

Subach FVPatterson GHManley SGillette JMLippincott-Schwartz JVerkhusha VV
Nat. Methods
2009 Feb

Patterson GHHirschberg KPolishchuk RSGerlich DPhair RDLippincott-Schwartz J
2008 Jun 13

Manley SGillette JMPatterson GHShroff HHess HFBetzig ELippincott-Schwartz J
Nat. Methods
2008 Feb

Lippincott-Schwartz JPatterson GH
Methods Cell Biol.

Patterson GH
Methods Mol. Biol.

Shaner NCPatterson GHDavidson MW
J. Cell. Sci.
2007 Dec 15

Betzig EPatterson GHSougrat RLindwasser OWOlenych SBonifacino JSDavidson MWLippincott-Schwartz JHess HF
2006 Sep 15

Chen YMacDonald PJSkinner JPPatterson GHMüller JD
Microsc. Res. Tech.
2006 Mar

Rossman JSStoicheva NGLangel FDPatterson GHLippincott-Schwartz JSchaefer BC
Mol. Biol. Cell
2006 May

Hodges MYikilmaz EPatterson GKasvosve IRouault TAGordeuk VRLoyevsky M
Mol. Biochem. Parasitol.
2005 Sep

Patterson GH
Nat. Biotechnol.
2004 Dec

Patterson GHLippincott-Schwartz J
2004 Apr

Lippincott-Schwartz JAltan-Bonnet NPatterson GH
Nat. Cell Biol.
2003 Sep

Lippincott-Schwartz JPatterson GH
2003 Apr 04

Patterson GHLippincott-Schwartz J
2002 Sep 13

Patterson GDay RNPiston D
J. Cell. Sci.
2001 Mar

Patterson GHPiston DWBarisas BG
Anal. Biochem.
2000 Sep 10

Patterson GHKnobel SMArkhammar PThastrup OPiston DW
Proc. Natl. Acad. Sci. U.S.A.
2000 May 09

Patterson GHPiston DW
Biophys. J.
2000 Apr