George Harold Patterson

George Patterson head shot
Senior Investigator

BG 13 RM 3E33 13 South Dr Bethesda MD 20814


George Patterson received a bachelor's degree for study in biology and research on the reproductive cycle of a freshwater mussel, Cyclonais tuberculata, from the University of North Alabama (Florence, Alabama) in 1992.  In 1993, he was accepted into the Interdisciplinary Graduate Program within the Vanderbilt School of Medicine, Vanderbilt University, Nashville, Tennessee. He joined the lab of David Piston in the Department of Molecular Physiology in 1994 and was awarded a PhD in 1999 for his study of glucose metabolism in pancreatic islets of Langerhans and quantitative fluorescence imaging using fluorescent proteins.  He accepted a post-doctoral position in the lab of Jennifer Lippincott-Schwartz in the Cell Biology and Metabolism Branch (National Institute of Child Health and Human Development, National Institutes of Health) in Bethesda, Maryland.  During his post-doctoral training, he developed reagents and techniques for fluorescence imaging in the study of secretory protein trafficking.  After 4 years, he accepted a staff scientist position at the NIH, where he continued work in protein trafficking, collaborative efforts with Eric Betzig and Harald Hess (Howard Hughes Medical Institute, Janelia Farm) in development of new fluorescence imaging techniques, and collaborative work with Vladislav Verkhusha (Albert Einstein School of Medicine, Bronx, New York) in development of new fluorescence imaging reagents.  He accepted a position in the National Institute of Biomedical Imaging and Bioengineering at the NIH in October 2009 where he continues work on fluorescent protein development and super-resolution imaging techniques for use in cell biology applications. In June 2021, he became a tenured senior investigator at NIBIB.

Research Interests

The NIBIB Section on Biophotonics develops probes and techniques for use in diffraction limited and sub-diffraction limited fluorescence imaging of cells and tissues. These include Photo-activated localization microscopy (PALM) and other molecular localization techniques which are based on imaging of single fluorescent molecules and Multi-focal Structured Illumination Microscopy (MSIM) which is based on exciting fluorophores with an array of diffraction-limited spots followed processing to achieve an approximately 2-fold improvement in resolution.  Major emphasis is placed on developing new and improving existing genetically encoded fluorescent proteins for use as markers and sensors.  This approach utilizes structure based mutagenesis followed by spectroscopy and imaging characterization of the variants.  Methods and technologies include multi-photon excitation microscopy, confocal microscopy, total internal reflection fluorescence (TIRF) microscopy, and widefield microscopy, single molecule imaging, fluorescence spectroscopy, and protein engineering.

Jacoby-Morris KPatterson GH
Methods Mol Biol

Zhao HWu DNguyen ALi YAdão RCValkov EPatterson GHPiszczek GSchuck P
2021 Jun 25

Shomron ONevo-Yassaf IAviad TYaffe YZahavi EEDukhovny APerlson EBrodsky IYeheskel APasmanik-Chor MMironov ABeznoussenko GVMironov AASklan EHPatterson GHYonemura YSannai MKaether CHirschberg K
J Cell Biol
2021 Jun 07

Zhao HWu DNguyen ALi YAdão RCValkov EPatterson GHPiszczek GSchuck P
2021 Feb 09

Rainey KHPatterson GH
Proc Natl Acad Sci U S A
2019 Jan 15

Guo MChandris PGiannini JPTrexler AJFischer RChen JVishwasrao HDRey-Suarez IWu YWu XWaterman CMPatterson GHUpadhyaya ATaraska JWShroff H
Nat Methods
2018 Jun

Salka KBhuvanendran SWilson KBozidis PMehta MRainey KSesaki HPatterson GHJaiswal JKColberg-Poley AM
Sci Rep
2017 Feb 02

Fu YWinter PWRojas RWang VMcAuliffe MPatterson GH
Proc Natl Acad Sci U S A
2016 Apr 19

Sengupta PSatpute-Krishnan PSeo AYBurnette DTPatterson GHLippincott-Schwartz J
Proc Natl Acad Sci U S A
2015 Dec 08

Long AHHaso WMShern JFWanhainen KMMurgai MIngaramo MSmith JPWalker AJKohler MEVenkateshwara VRKaplan RNPatterson GHFry TJOrentas RJMackall CL
Nat Med
2015 Jun

Colberg-Poley AMPatterson GHSalka KBhuvanendran SYang DJaiswal JK
Med Microbiol Immunol
2015 Jun

Winter PWYork AGNogare DDIngaramo MChristensen RChitnis APatterson GHShroff H
2014 Sep 20

Zhao HMa JIngaramo MAndrade EMacDonald JRamsay GPiszczek GPatterson GHSchuck P
Anal Chem
2014 Sep 16

Bhuvanendran SSalka KRainey KSreetama SCWilliams ELeeker MPrasad VBoyd JPatterson GHJaiswal JKColberg-Poley AM
2014 Apr 09

Ingaramo MYork AGWawrzusin PMilberg OHong AWeigert RShroff HPatterson GH
Proc Natl Acad Sci U S A
2014 Apr 08

Ingaramo MYork AGHoogendoorn EPostma MShroff HPatterson GH
2014 Mar 17

Zhao HBerger AJBrown PHKumar JBalbo AMay CACasillas ELaue TMPatterson GHMayer MLSchuck P
J Gen Physiol
2013 Jun

Yang JSingh VCha BChen TESarker RMurtazina RJin SZachos NCPatterson GHTse CMKovbasnjuk OLi XDonowitz M
J Biol Chem
2013 Jun 07

Zhao HBerger AJBrown PHKumar JBalbo AMay CACasillas ELaue TMPatterson GHMayer MLSchuck P
J Gen Physiol
2012 May

Datta SRPatterson GH
Curr Opin Neurobiol
2012 Feb

Subach OMPatterson GHTing LMWang YCondeelis JSVerkhusha VV
Nat Methods
2011 Jul 31

Subach FVPatterson GHRenz MLippincott-Schwartz JVerkhusha VV
J Am Chem Soc
2010 May 12

Patterson GH
Semin Cell Dev Biol
2009 Oct

Subach FVPatterson GHManley SGillette JMLippincott-Schwartz JVerkhusha VV
Nat Methods
2009 Feb

Patterson GHHirschberg KPolishchuk RSGerlich DPhair RDLippincott-Schwartz J
2008 Jun 13

Manley SGillette JMPatterson GHShroff HHess HFBetzig ELippincott-Schwartz J
Nat Methods
2008 Feb

Lippincott-Schwartz JPatterson GH
Methods Cell Biol

Patterson GH
Methods Mol Biol

Shaner NCPatterson GHDavidson MW
J Cell Sci
2007 Dec 15

Betzig EPatterson GHSougrat RLindwasser OWOlenych SBonifacino JSDavidson MWLippincott-Schwartz JHess HF
2006 Sep 15

Chen YMacDonald PJSkinner JPPatterson GHMüller JD
Microsc Res Tech
2006 Mar

Rossman JSStoicheva NGLangel FDPatterson GHLippincott-Schwartz JSchaefer BC
Mol Biol Cell
2006 May

Patterson GH
Nat Biotechnol
2004 Dec

Patterson GHLippincott-Schwartz J
2004 Apr

Lippincott-Schwartz JAltan-Bonnet NPatterson GH
Nat Cell Biol
2003 Sep

Lippincott-Schwartz JPatterson GH
2003 Apr 04

Patterson GHLippincott-Schwartz J
2002 Sep 13