In Memoriam - George Harold Patterson - (1970-2021)

George Patterson head shot
George
Patterson
Ph.D.
Senior Investigator

BG 13 RM 3E33 13 South Dr Bethesda MD 20814

Biography

George Harold Patterson, Ph.D., was a Senior Investigator in NIBIB, where he served as Chief of the Section on Biophotonics. George’s research focused on the development of probes and techniques for diffraction limited and sub-diffraction limited fluorescence imaging of cells and tissues. These approaches have included photo-activated localization microscopy (PALM); other molecular localization techniques based on imaging of single fluorescent molecules; and multi-focal structured illumination microscopy (MSIM) techniques, which are based on exciting fluorophores with an array of diffraction-limited focused beams, followed by image processing to improve spatial resolution.

George’s career started with his bachelor's degree from the University of North Alabama (Florence, Alabama) in 1992, which included a biology research project on the reproductive cycle of a freshwater mussel, Cyclonais tuberculata. In 1993, George was accepted into the Interdisciplinary Graduate Program within the Vanderbilt School of Medicine, Vanderbilt University, Nashville, Tennessee, where he joined the lab of Prof. David Piston in the Department of Molecular Physiology in 1994. George was awarded a PhD in 1999 for his study of glucose metabolism in pancreatic islets of Langerhans, which incorporated quantitative fluorescence imaging using fluorescent proteins. He then accepted a post-doctoral position in the laboratory of Dr. Jennifer Lippincott-Schwartz in the Cell Biology and Metabolism Branch, in the intramural program of the National Institute of Child Health and Human Development, where he developed reagents and techniques for fluorescence imaging in the study of secretory protein trafficking. Four years later, he accepted a staff scientist position in Jennifer’s laboratory, where he continued work in protein trafficking. During this time, George collaborated with Dr. Eric Betzig, Dr. Harald Hess at the Howard Hughes Medical Institute, Janelia Farm, and the team developed the new PALM fluorescence imaging technique. George’s work on photoactivatable genetically encoded proteins made an important contribution to the work that led to Dr. Eric Betzig’s Nobel Prize in Chemistry in 2014.

In late 2009, George accepted an Investigator appointment in the National Institute of Biomedical Imaging and Bioengineering, where he worked on the development fluorescent proteins, as well as on super-resolution imaging techniques for use in cell biology. While in NIBIB, George made major contributions to create both novel and improved genetically encoded fluorescent proteins for use as markers and sensors. His approach utilized structure-based mutagenesis followed by spectroscopy and imaging characterization of the variants. His broad range of technique development included multi-photon excitation microscopy, confocal microscopy, total internal reflection fluorescence (TIRF) microscopy, wide-field microscopy, single molecule imaging, fluorescence spectroscopy, and protein engineering.

Notably, George created a friendly laboratory with a high degree of trainee diversity. George will be greatly missed by his colleagues in NIBIB and around the NIH. George is survived by his wife Susanne Neumann, and children Isabella (11) and Max (9).

Research Interests

The NIBIB Section on Biophotonics develops probes and techniques for use in diffraction limited and sub-diffraction limited fluorescence imaging of cells and tissues. These include Photo-activated localization microscopy (PALM) and other molecular localization techniques which are based on imaging of single fluorescent molecules and Multi-focal Structured Illumination Microscopy (MSIM) which is based on exciting fluorophores with an array of diffraction-limited spots followed processing to achieve an approximately 2-fold improvement in resolution.  Major emphasis is placed on developing new and improving existing genetically encoded fluorescent proteins for use as markers and sensors.  This approach utilizes structure based mutagenesis followed by spectroscopy and imaging characterization of the variants.  Methods and technologies include multi-photon excitation microscopy, confocal microscopy, total internal reflection fluorescence (TIRF) microscopy, and widefield microscopy, single molecule imaging, fluorescence spectroscopy, and protein engineering.

Publications
Jacoby-Morris KPatterson GH
Methods Mol Biol
2021

Zhao HWu DNguyen ALi YAdão RCValkov EPatterson GHPiszczek GSchuck P
iScience
2021 Jun 25

Shomron ONevo-Yassaf IAviad TYaffe YZahavi EEDukhovny APerlson EBrodsky IYeheskel APasmanik-Chor MMironov ABeznoussenko GVMironov AASklan EHPatterson GHYonemura YSannai MKaether CHirschberg K
J Cell Biol
2021 Jun 07

Zhao HWu DNguyen ALi YAdão RCValkov EPatterson GHPiszczek GSchuck P
bioRxiv
2021 Feb 09

Rainey KHPatterson GH
Proc Natl Acad Sci U S A
2019 Jan 15

Guo MChandris PGiannini JPTrexler AJFischer RChen JVishwasrao HDRey-Suarez IWu YWu XWaterman CMPatterson GHUpadhyaya ATaraska JWShroff H
Nat Methods
2018 Jun

Salka KBhuvanendran SWilson KBozidis PMehta MRainey KSesaki HPatterson GHJaiswal JKColberg-Poley AM
Sci Rep
2017 Feb 02

Fu YWinter PWRojas RWang VMcAuliffe MPatterson GH
Proc Natl Acad Sci U S A
2016 Apr 19

Sengupta PSatpute-Krishnan PSeo AYBurnette DTPatterson GHLippincott-Schwartz J
Proc Natl Acad Sci U S A
2015 Dec 08


Long AHHaso WMShern JFWanhainen KMMurgai MIngaramo MSmith JPWalker AJKohler MEVenkateshwara VRKaplan RNPatterson GHFry TJOrentas RJMackall CL
Nat Med
2015 Jun

Colberg-Poley AMPatterson GHSalka KBhuvanendran SYang DJaiswal JK
Med Microbiol Immunol
2015 Jun

Winter PWYork AGNogare DDIngaramo MChristensen RChitnis APatterson GHShroff H
Optica
2014 Sep 20

Zhao HMa JIngaramo MAndrade EMacDonald JRamsay GPiszczek GPatterson GHSchuck P
Anal Chem
2014 Sep 16

Bhuvanendran SSalka KRainey KSreetama SCWilliams ELeeker MPrasad VBoyd JPatterson GHJaiswal JKColberg-Poley AM
Viruses
2014 Apr 09

Ingaramo MYork AGWawrzusin PMilberg OHong AWeigert RShroff HPatterson GH
Proc Natl Acad Sci U S A
2014 Apr 08

Ingaramo MYork AGHoogendoorn EPostma MShroff HPatterson GH
Chemphyschem
2014 Mar 17


Zhao HBerger AJBrown PHKumar JBalbo AMay CACasillas ELaue TMPatterson GHMayer MLSchuck P
J Gen Physiol
2013 Jun

Yang JSingh VCha BChen TESarker RMurtazina RJin SZachos NCPatterson GHTse CMKovbasnjuk OLi XDonowitz M
J Biol Chem
2013 Jun 07

Zhao HBerger AJBrown PHKumar JBalbo AMay CACasillas ELaue TMPatterson GHMayer MLSchuck P
J Gen Physiol
2012 May

Datta SRPatterson GH
Curr Opin Neurobiol
2012 Feb

Subach OMPatterson GHTing LMWang YCondeelis JSVerkhusha VV
Nat Methods
2011 Jul 31


Subach FVPatterson GHRenz MLippincott-Schwartz JVerkhusha VV
J Am Chem Soc
2010 May 12


Patterson GH
Semin Cell Dev Biol
2009 Oct

Subach FVPatterson GHManley SGillette JMLippincott-Schwartz JVerkhusha VV
Nat Methods
2009 Feb

Patterson GHHirschberg KPolishchuk RSGerlich DPhair RDLippincott-Schwartz J
Cell
2008 Jun 13


Manley SGillette JMPatterson GHShroff HHess HFBetzig ELippincott-Schwartz J
Nat Methods
2008 Feb

Lippincott-Schwartz JPatterson GH
Methods Cell Biol
2008

Patterson GH
Methods Mol Biol
2007

Shaner NCPatterson GHDavidson MW
J Cell Sci
2007 Dec 15

Betzig EPatterson GHSougrat RLindwasser OWOlenych SBonifacino JSDavidson MWLippincott-Schwartz JHess HF
Science
2006 Sep 15

Chen YMacDonald PJSkinner JPPatterson GHMüller JD
Microsc Res Tech
2006 Mar

Rossman JSStoicheva NGLangel FDPatterson GHLippincott-Schwartz JSchaefer BC
Mol Biol Cell
2006 May

Patterson GH
Nat Biotechnol
2004 Dec

Patterson GHLippincott-Schwartz J
Methods
2004 Apr

Lippincott-Schwartz JAltan-Bonnet NPatterson GH
Nat Cell Biol
2003 Sep

Lippincott-Schwartz JPatterson GH
Science
2003 Apr 04

Patterson GHLippincott-Schwartz J
Science
2002 Sep 13