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Biophysical Methods for Protein Interactions Zoom Lectures

Lectures will be streamed on zoom which are open to NIH members (registration required)

Please register with your NIH email.

 

Monday 5/15

08:30-09:15

Basic Principles of AUC

Joy Zhao, Ph.D., Laboratory of Dynamics of Macromolecular Assembly, NIBIB, NIH

09:15-10:00

Introduction to SV-AUC

Peter Schuck, Ph.D., Laboratory of Dynamics of Macromolecular Assembly, NIBIB, NIH

10:15-11:15 

Introduction to ITC

Joy Zhao, Ph.D., Laboratory of Dynamics of Macromolecular Assembly, NIBIB, NIH



 

Tuesday, 5/16

08:30-09:15

Introduction to Small-Angle X-Ray Scattering

Alexander Grishaev, Ph.D., UMD/NIST Institute for Bioscience & Biotechnology Research

09:15-10:00

Microfluidic Diffusional Sizing

Sandro Keller, Ph.D., Institute of Molecular Biosciences, University of Graz, Austria



 

Wednesday, 5/17

08:30-09:15

Hydrodynamic Parameter Estimates from Structures

Rodolfo Ghirlando, Ph.D., Laboratory of Molecular Biology, NIDDK, NIH

09:15-10:00

Introduction to Mass Photometry

Greg Piszczek, Ph.D., Biophysics Core Facility, NHLBI, NIH

10:15-11:00

SPR Analysis and Binding Site Distributions (EVILFIT)

Joy Zhao, Ph.D., Laboratory of Dynamics of Macromolecular Assembly, NIBIB, NIH



 

Thursday, 5/18

08:30-09:15

Static and Dynamic Light Scattering

Rodolfo Ghirlando, Ph.D., Laboratory of Molecular Biology, NIDDK, NIH

09:15-10:00

Introduction to Fluorescence Spectroscopy

Greg Piszczek, Ph.D., Biophysics Core Facility, NHLBI, NIH

 

Friday, 5/19

08:30-09:15

Global Multi-Method Analysis (GMMA)

Peter Schuck, Ph.D., Laboratory of Dynamics of Macromolecular Assembly, NIBIB, NIH

09:15-10:00

Determination of molecular mass and subunit arrangement of large protein assemblies by scanning transmission electron microscopy

Richard Leapman, Ph.D., Laboratory of Dynamics of Macromolecular Assembly, NIBIB, NIH

10:15 – 11:00

Introduction to Sedimentation Equilibrium (SE-AUC)

Rodolfo Ghirlando, Ph.D., Laboratory of Molecular Biology, NIDDK, NIH