Micro Analytical Immunochemistry

Heather R Kalish

Heather Kalish

Ph.D.

Chemist

301-435-5498

kalishh@mail.nih.gov

The Micro Analytical Immunochemistry Unit of the BEPS Shared Resource specializes in the following:

Micro-immunoaffinity capillary electrophoresis to quantify analyte concentrations in biological samples
Development of novel immunoassays for microliter and sub-microliter biological fluid and tissue samples
SELEX via capillary electrophoresis to generate aptamers with specificity for protein targets
Multianalyte ELISA arrays using Quansys^TM imaging system
MALDI-TOF analysis of proteins, antibodies and other biological molecules
Characterization of biological molecule interactions using Surface Plasmon Resonance (SPR)
Trace metal analysis in biological samples, via Inductively-Coupled-Plasma Optical Emission Spectrometry (ICP-OES)

We are located in Building 13 on the NIH campus.

Lab Resources

This is an image of a multiplex ELISA array with 16 cytokines per well on a 96 well plate

The mission of the lab is to get as much information out of as little sample as possible. The technology is applicable to all biological samples, from serum and plasma, to cells and tissue.

Develop novel immunoassays to quantitate analyte concentrations
Use immunoaffinity capillary electrophoresis to extract analytes from:
- tissue sample
- splasma and other biological fluids
- cells and cell supernatant

This is an image of a micralyne double T chip staged on a Micralyne microchip capillary electrophoresis unit

Identify and quantify cytokines, chemokines, neuropeptides, hormones, and other analytes in microliter and sub-microliter samples
Use QuansysTM imaging system to screen for up to 16 cytokines from a single sample using a multiplex ELISA
Determine the mass of proteins, antibodies and other biological complexes using MALDI-TOF
Screen for high affinity aptamers to specific protein targets using CE-based SELEX
Characterize biological molecular interactions using Surface Plasmon Resonance (SPR)

Surface Plasmon Resonance (SPR) Technology

Characterization of biological molecule interactions using Surface Plasmon Resonance (SPR) optical biosensor technology (Biacore3000)

This is an illustration of a surface plasmon resonance sensor chip containing carboxymethylated dextran attached to a gold surface

Interactions include, but are not limited to:
- Protein-protein, protein-peptide, protein-small molecules, protein-whole cells
- Nucleic acid-protein, nucleic acid-small molecules
- Liposome-protein, liposome-small molecules
Information yield
- Yes or no binding
- Binding specificity
- Binding affinity (KD - Thermodynamic constant of binding\
- Binding kinetics (kon, koff -kinetic constants of association and dissociation).

Trace Metal Analysis in biological samples (down to te ppb level), via ICP-OES

Staff

Heather R Kalish

Publications

D614G Spike Variant Does Not Alter IgG, IgM, or IgA Spike Seroassay Performance.

Klumpp-Thomas CKalish HHicks JMehalko JDrew MMemoli MJHall MDEsposito DSadtler K

medRxiv

2020 Jul 10

Serologic cross-reactivity of SARS-CoV-2 with endemic and seasonal Betacoronaviruses.

Hicks JKlumpp-Thomas CKalish HShunmugavel AMehalko JDenson JPSnead KDrew MCorbett KGraham BHall MDEsposito DSadtler K

medRxiv

2020 Jun 23

Standardization of enzyme-linked immunosorbent assays for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling.

Klumpp-Thomas CKalish HDrew MHunsberger SSnead KFay MPMehalko JShunmugavel AWall VFrank PDenson JPHong MGulten GMessing SHicks JMichael SGillette WHall MDMemoli MEsposito DSadtler K

medRxiv

2020 May 25

Glutathione-Responsive Self-Assembled Magnetic Gold Nanowreath for Enhanced Tumor Imaging and Imaging-Guided Photothermal Therapy.

Liu YYang ZHuang XYu GWang SZhou ZShen ZFan WLiu YDavisson MKalish HNiu GNie ZChen X

ACS Nano

2018 Aug 28

Suppressing Nanoparticle-Mononuclear Phagocyte System Interactions of Two-Dimensional Gold Nanorings for Improved Tumor Accumulation and Photothermal Ablation of Tumors.