1. Labs @ NIBIB
  2. Trans-NIH Shared Resource on Biomedical Engineering and Physical Science (BEPS)
  3. Micro Analytical Immunochemistry

Micro Analytical Immunochemistry

Our unit employs a variety of techniques to isolate, identify and quantitate proteins, peptides, lipids, antibodies and other biological molecules of interest. The unit currently has a range of active projects using MALDI-TOF, single and multiplex ELISA and ICP-OES spectroscopy, including:

  • Using a QuansysTM imaging system to screen for up to 16 analytes from a single 50 mL sample using a multiplex ELISA
  • Automated ELISA system currently being used to determine seropositivity in whole blood, serum and plasma samples, including nucleocapsid testing to discriminate between native or immunized seropositivity. 96 and 384 well formats can be run.
  • Determining the mass of biological and chemical compounds using MALDI-TOF
  • MALDI-TOF imaging of frozen tissue samples to provide spatial resolution of molecules of interest, extracting unknown samples from the imaged tissues and identifying proteins, peptides and lipids using LC/MS.
  • Quantifying trace metals in chemical and biological samples down to a ppb level

We are located in Building 13 on the NIH campus.

Lab Resources

  • Multianalyte ELISA arrays using QuansysTM imaging system
  • Automated ELISA assays for SARS-COV-2 seropositivity
  • MALDI-TOF analysis of chemical and biological molecules
  • MALDI-TOF imaging of tissue samples
  • Surface liquid extraction (SELEX) from tissue samples for analysis and identification of unknown proteins, peptides, and lipids using LC/MS
  • Trace metal analysis in biological samples, via Inductively-Coupled-Plasma Optical Emission Spectrometry (ICP-OES)
  • Micro-immunoaffinity capillary electrophoresis to quantify analyte concentrations in biological samples

Staff

Publications

Undiagnosed COVID-19 in households with a child with mitochondrial disease.
Gordon-Lipkin EMMarcum CKruk SThompson EKelly SEMKalish HSadtler KMcGuire PJ
medRxiv
2022 Mar 23

Robust, persistent adaptive immune responses to SARS-CoV-2 in the oropharyngeal lymphoid tissue of children.
Manthiram KXu QMilanez-Almeida PMartins ARadtke AHoehn KChen JLiu CTang JGrubbs GStein SRamelli SKabat JBehzadpour HKarkanitsa MSpathies JKalish HKardava LKirby MCheung FPreite SDuncker PRomero NPreciado DGitman LKoroleva GSmith GShaffer AMcBain IPittaluga SGermain RApps RSadtler KMoir SChertow DKleinstein SKhurana STsang JMudd PSchwartzberg P
Res Sq
2022 Mar 23

Lung epithelial and endothelial damage, loss of tissue repair, inhibition of fibrinolysis, and cellular senescence in fatal COVID-19.
D'Agnillo FWalters KAXiao YSheng ZMScherler KPark JGygli SRosas LASadtler KKalish HBlatti CAZhu RGatzke LBushell CMemoli MJO'Day SJFischer TDHammond TCLee RCCash JCPowers MEO'Keefe GEButnor KJRapkiewicz AVTravis WDLayne SPKash JCTaubenberger JK
Sci Transl Med
2021 Nov 17

SARS-CoV-2 Seroprevalence and Drug Use in Trauma Patients from Six Sites in the United States.
Ngo TBKarkanitsa MAdusei KMGraham LARicotta EEDarrah JRBlomberg RDSpathies JPauly KJKlumpp-Thomas CTravers JMehalko JDrew MHall MDMemoli MJEsposito DKozar RAGriggs CCunningham KWSchulman CICrandall MNeavyn MDorfman JDLai JTWhitehill JMBabu KMMohr NMVan Heukelom JFell JCRooke WKalish HThomas FDSadtler K
medRxiv
2021 Aug 11

Undiagnosed SARS-CoV-2 seropositivity during the first 6 months of the COVID-19 pandemic in the United States.
Kalish HKlumpp-Thomas CHunsberger SBaus HAFay MPSiripong NWang JHicks JMehalko JTravers JDrew MPauly KSpathies JNgo TAdusei KMKarkanitsa MCroker JALi YGraubard BICzajkowski LBelliveau OChairez CSnead KRFrank PShunmugavel AHan AGiurgea LTRosas LABean RAthota RCervantes-Medina AGouzoulis MHeffelfinger BValenti SCaldararo RKolberg MMKelly ASimon RShafiq SWall VReed SFord EWLokwani RDenson JPMessing SMichael SGGillette WKimberly RPReis SEHall MDEsposito DMemoli MJSadtler K
Sci Transl Med
2021 Jul 07

Serologic Cross-Reactivity of SARS-CoV-2 with Endemic and Seasonal Betacoronaviruses.
Hicks JKlumpp-Thomas CKalish HShunmugavel AMehalko JDenson JPSnead KRDrew MCorbett KSGraham BSHall MDMemoli MJEsposito DSadtler K
J Clin Immunol
2021 Jul

Mapping a Pandemic: SARS-CoV-2 Seropositivity in the United States.
Kalish HKlumpp-Thomas CHunsberger SBaus HAFay MPSiripong NWang JHicks JMehalko JTravers JDrew MPauly KSpathies JNgo TAdusei KMKarkanitsa MCroker JALi YGraubard BICzajkowski LBelliveau OChairez CSnead KFrank PShunmugavel AHan AGiurgea LTRosas LABean RAthota RCervantes-Medina AGouzoulis MHeffelfinger BValenti SCaldararo RKolberg MMKelly ASimon RShafiq SWall VReed SFord EWLokwani RDenson JPMessing SMichael SGGillette WKimberly RPReis SEHall MDEsposito DMemoli MJSadtler K
medRxiv
2021 Jan 31

Standardization of ELISA protocols for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling.
Klumpp-Thomas CKalish HDrew MHunsberger SSnead KFay MPMehalko JShunmugavel AWall VFrank PDenson JPHong MGulten GMessing SHicks JMichael SGillette WHall MDMemoli MJEsposito DSadtler K
Nat Commun
2021 Jan 04

Effect of D614G Spike Variant on Immunoglobulin G, M, or A Spike Seroassay Performance.
Klumpp-Thomas CKalish HHicks JMehalko JDrew MMemoli MJHall MDEsposito DSadtler K
J Infect Dis
2021 Mar 03

D614G Spike Variant Does Not Alter IgG, IgM, or IgA Spike Seroassay Performance.
Klumpp-Thomas CKalish HHicks JMehalko JDrew MMemoli MJHall MDEsposito DSadtler K
medRxiv
2020 Jul 10

Serologic cross-reactivity of SARS-CoV-2 with endemic and seasonal Betacoronaviruses.
Hicks JKlumpp-Thomas CKalish HShunmugavel AMehalko JDenson JPSnead KDrew MCorbett KGraham BHall MDMemoli MJEsposito DSadtler K
medRxiv
2020 Jun 23

Standardization of enzyme-linked immunosorbent assays for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling.
Klumpp-Thomas CKalish HDrew MHunsberger SSnead KFay MPMehalko JShunmugavel AWall VFrank PDenson JPHong MGulten GMessing SHicks JMichael SGillette WHall MDMemoli MEsposito DSadtler K
medRxiv
2020 May 25

Glutathione-Responsive Self-Assembled Magnetic Gold Nanowreath for Enhanced Tumor Imaging and Imaging-Guided Photothermal Therapy.
Liu YYang ZHuang XYu GWang SZhou ZShen ZFan WLiu YDavisson MKalish HNiu GNie ZChen X
ACS Nano
2018 Aug 28

Suppressing Nanoparticle-Mononuclear Phagocyte System Interactions of Two-Dimensional Gold Nanorings for Improved Tumor Accumulation and Photothermal Ablation of Tumors.
Liu YWang ZLiu YZhu GJacobson OFu XBai RLin XLu NYang XFan WSong JWang ZYu GZhang FKalish HNiu GNie ZChen X
ACS Nano
2017 Oct 24

Importance of the REM (Ras exchange) domain for membrane interactions by RasGRP3.
Czikora AKedei NKalish HBlumberg PM
Biochim Biophys Acta Biomembr
2017 Dec

Magneto-Plasmonic Janus Vesicles for Magnetic Field-Enhanced Photoacoustic and Magnetic Resonance Imaging of Tumors.
Liu YYang XHuang ZHuang PZhang YDeng LWang ZZhou ZLiu YKalish HKhachab NMChen XNie Z
Angew Chem Int Ed Engl
2016 Dec 05

Measurement of Inflammatory Chemokines in Micro-dissected Tissue Biopsy Samples by Chip-Based Immunoaffinity Capillary Electrophoresis.
Phillips TMWellner EMcMohan SKalish H
Methods Mol Biol
2016

Obstructive sleep apnea is a predictor of abnormal glucose metabolism in chronically sleep deprived obese adults.
Cizza GPiaggi PLucassen EAde Jonge LWalter MMattingly MSKalish HCsako GRother KI
PLoS One
2013

Cytokine analysis by immunoaffinity capillary electrophoresis.
Mendonca MKalish H
Methods Mol Biol
2013

Assessment of chemokine profiles in human skin biopsies by an immunoaffinity capillary electrophoresis chip.
Kalish HPhillips TM
Methods
2012 Feb

Receptor affinity CE for measuring bioactive inflammatory cytokines in human skin biopsies.
Phillips TMKalish HWellner E
Electrophoresis
2009 Nov

Analysis of neurotrophins in human serum by immunoaffinity capillary electrophoresis (ICE) following traumatic head injury.
Kalish HPhillips TM
J Chromatogr B Analyt Technol Biomed Life Sci
2010 Jan 15

Application of immunoaffinity capillary electrophoresis to the measurements of secreted cytokines by cultured astrocytes.
Kalish HPhillips TM
J Sep Sci
2009 May

A chip-based immunoaffinity capillary electrophoresis assay for assessing hormones in human biological fluids.
Wellner EFKalish H
Electrophoresis
2008 Aug

Color transformation and fluorescence of Prussian blue-positive cells: implications for histologic verification of cells labeled with superparamagnetic iron oxide nanoparticles.
Frank JAKalish HJordan EKAnderson SAPawelczyk EArbab AS
Mol Imaging
2007

Solution 1H NMR study of the accommodation of the side chain of n-butyl-etiohemin-I incorporated into the active site of cyano-metmyoglobin.
Bondarenko VWang JKalish HBalch ALLa Mar GN
J Biol Inorg Chem
2005 May

Comparison of transfection agents in forming complexes with ferumoxides, cell labeling efficiency, and cellular viability.
Arbab ASYocum GTWilson LBParwana AJordan EKKalish HFrank JA
Mol Imaging
2004 Jan

Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI.
Arbab ASYocum GTKalish HJordan EKAnderson SAKhakoo AYRead EJFrank JA
Blood
2004 Aug 15

Facile ring opening of iron(III) and iron(II) complexes of meso-amino-octaethylporphyrin by dioxygen.
Rath SPKalish HLatos-Grazyński LOlmstead MMBalch AL
J Am Chem Soc
2004 Jan 21

Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging.
Arbab ASBashaw LAMiller BRJordan EKLewis BKKalish HFrank JA
Radiology
2003 Dec

Heme cleavage with remarkable ease: paramagnetic intermediates formed by aerobic oxidation of a meso-amino-substituted iron porphyrin.
Kalish HLee HMOlmstead MMLatos-Grazyński LRath SPBalch AL
J Am Chem Soc
2003 Apr 23

meso Substituent effects on the geometric and electronic structures of high-spin and low-spin iron(III) complexes of mono-meso-substituted octaethylporphyrins.
Kalish HCamp JEStepień MLatos-Grazyński LOlmstead MMBalch AL
Inorg Chem
2002 Feb 25