Micro Analytical Immunochemistry

The mission of the lab is to get as much information out of as little sample as possible. The technology is applicable to all biological samples, from serum and plasma, to cells and tissue.

• Develop novel immunoassays to quantitate analyte concentrations
• Use immunoaffinity capillary electrophoresis to extract analytes from:
  • tissue sample
  • splasma and other biological fluids
  • cells and cell supernatant

• Identify and quantify cytokines, chemokines, neuropeptides, hormones, and other analytes in microliter and sub-microliter samples
• Use QuansysTM imaging system to screen for up to 16 cytokines from a single sample using a multiplex ELISA
• Determine the mass of proteins, antibodies and other biological complexes using MALDI-TOF
• Screen for high affinity aptamers to specific protein targets using CE-based SELEX
• Characterize biological molecular interactions using Surface Plasmon Resonance (SPR)

Surface Plasmon Resonance (SPR) Technology

Characterization of biological molecule interactions using Surface Plasmon Resonance (SPR) optical biosensor technology (Biacore3000)

• Interactions include, but are not limited to:
  • Protein-protein, protein-peptide, protein-small molecules, protein-whole cells
  • Nucleic acid-protein, nucleic acid-small molecules
  • Liposome-protein, liposome-small molecules
• Information yield
  • Yes or no binding
  • Binding specificity
  • Binding affinity (KD - Thermodynamic constant of binding\
  • Binding kinetics (kon, koff -kinetic constants of association and dissociation).

Trace Metal Analysis in biological samples (down to te ppb level), via ICP-OES