Richard D Leapman, Ph.D.

portrait of Richard Leapman in lab
Richard
Leapman
Ph.D.
Scientific Director

BG 13 RM 3E63 13 South Dr Bethesda MD 20814

Biography

Dr. Leapman obtained B.A. and M.A. in Natural Sciences, and Ph.D. in physics from the University of Cambridge, UK. He then trained as a postdoctoral fellow in the Department of Materials at the University of Oxford, UK, and in the Department of Applied and Engineering Physics at Cornell University, NY, where he contributed to the development of electron spectroscopy for the nanoscale characterization of materials.  He subsequently moved to NIH to develop methods based on scanning transmission electron microscopy and electron spectroscopy to determine the structure and chemical composition of cells and supramolecular assemblies. Dr. Leapman was elected a Fellow of the Microscopy Society of America in 2011.  He is currently Editor of the Journal of Microscopy (Oxford), a member of the editorial boards of other microscopy and nanotechnology journals, and has served on numerous national scientific advisory committees.  Dr. Leapman is Chief of NIBIB’s Laboratory of Cellular Imaging and Macromolecular Biophysics, while also serving as NIBIB’s Scientific Director since 2006.

Research Interests

The Section on Cellular and Supramolecular Structure and Function develops new methods based on electron microscopy and related techniques. Our aim is to expand knowledge about complex biological and disease processes, as well as to characterize morphologically the action of diagnostic markers and therapeutic agents in cells. The nanometer scale of biological electron microscopy lies between the realms of live-cell optical microscopy and atomic-scale structural tools of x-ray crystallography and NMR spectroscopy, both of which—unlike EM—require extraction and purification of cellular components. Our approach also complements cryo_EM of isolated frozen-hydrated supramolecular assemblies, which is difficult to apply to intact eukaryotic cells and tissues. We focus on the development of alternative quantitative imaging modes based on detection of elastic and inelastic electron scattering. Current research in the Section Includes development of techniques for (i) determining the tertiary and quaternary structures of macromolecular assemblies, (ii) visualizing 3D ultrastructure, (iii) mapping the elemental composition of subcellular compartments quantitatively, and (iv) studying bionanoparticles and their interactions with cells. We are applying these methods to structural biology, cellular biology, neurobiology, cancer biology, and nanomedicine.

Our group has been particularly active in developing the techniques of electron energy loss spectroscopy (EELS) and combining it with scanning transmission electron microscopy (STEM) to provide an unprecedented high spatial resolution for nanoanalysis of biological structures. We have devised new methods for quantifying both elemental and chemical information obtained from inelastic electron scattering. Recently, our group has extended the techniques of STEM and energy-filtered electron tomography to obtain three-dimensional structural and compositional information about cellular components.

Publications
Storrie BRhee SWPokrovskaya IDLing KVedanaparti YAronova MStalker TJBrass LFLeapman R
Blood
2019 Nov 13

Chen XWinters CCrocker VLazarou MSousa AALeapman RDReese TS
Front Neuroanat
2018

Pokrovskaya IDJoshi STobin MDesai RAronova MAKamykowski JAZhang GWhiteheart SWLeapman RDStorrie B
Blood Adv
2018 Nov 13


Shen ZSong JZhou ZYung BCAronova MALi YDai YFan WLiu YLi ZRuan HLeapman RDLin LNiu GChen XWu A
Adv. Mater. Weinheim
2018 Jul 04

McBride ELRao AZhang GHoyne JDCalco GNKuo BCHe QPrince AAPokrovskaya IDStorrie BSousa AAAronova MALeapman RD
J. Struct. Biol.
2018 Jun



Sweeney AMFleming KEMcCauley JPRodriguez MFMartin ETSousa AALeapman RDScimemi A
Sci Rep
2017 Mar 03

Lu NHuang PFan WWang ZLiu YWang SZhang GHu JLiu WNiu GLeapman RDLu GChen X
Biomaterials
2017 May

Zhu LAlmaça JDadi PKHong HSakamoto WRossi MLee RJVierra NCLu HCui YMcMillin SMPerry NAGurevich VVLee AKuo BLeapman RDMatschinsky FMDoliba NMUrs NMCaron MGJacobson DACaicedo AWess J
Nat Commun
2017 Feb 01

Yadav SWilliamson JKAronova MAPrince AAPokrovskaya IDLeapman RDStorrie B
Platelets
2017 Jun

Bryant LHKim SJHobson MMilo BKovacs ZIJikaria NLewis BKAronova MASousa AAZhang GLeapman RDFrank JA
Nanomedicine
2017 Feb

Guay MDCzaja WAronova MALeapman RD
Sci Rep
2016 Jun 13

Zhu GLiu YYang XKim YHZhang HJia RLiao HSJin ALin JAronova MLeapman RNie ZNiu GChen X
Nanoscale
2016 Mar 28

Sousa AAHassan SAKnittel LLBalbo AAronova MABrown PHSchuck PLeapman RD
Nanoscale
2016 Mar 28

Pokrovskaya IDAronova MAKamykowski JAPrince AAHoyne JDCalco GNKuo BCHe QLeapman RDStorrie B
J. Thromb. Haemost.
2016 Mar

Chen XLevy JMHou AWinters CAzzam RSousa AALeapman RDNicoll RAReese TS
Proc. Natl. Acad. Sci. U.S.A.
2015 Dec 15

Chen RJZhang GGarfield SHShi YJChen KGRobey PGLeapman RD
PLoS ONE
2015


Pfeifer CRShomorony AAronova MAZhang GCai TXu HNotkins ALLeapman RD
J. Struct. Biol.
2015 Jan

Pothayee NChen DYAronova MAQian CBouraoud NDodd SLeapman RDKoretsky AP
J Mater Chem B
2014

Graydon CWZhang JOesch NWSousa AALeapman RDDiamond JS
J. Neurosci.
2014 Jul 02

Bhirde AAChikkaveeraiah BVSrivatsan ANiu GJin AJKapoor AWang ZPatel SPatel VGorbach AMLeapman RDGutkind JSHight Walker ARChen X
ACS Nano
2014 May 27


Huang PLin JLi WRong PWang ZWang SWang XSun XAronova MNiu GLeapman RDNie ZChen X
Angew. Chem. Int. Ed. Engl.
2013 Dec 23

Abreu FSousa AAAronova MAKim YCox DLeapman RDAndrade LRKachar BBazylinski DALins U
J. Struct. Biol.
2013 Feb




Norlin NHellberg MFilippov ASousa AAGröbner GLeapman RDAlmqvist NAntzutkin ON
J. Struct. Biol.
2012 Oct


Fera ADosemeci ASousa AAYang CLeapman RDReese TS
J. Comp. Neurol.
2012 Dec 15

Bhirde AAKapoor ALiu GIglesias-Bartolome RJin AZhang GXing RLee SLeapman RDGutkind JSChen X
ACS Nano
2012 Jun 26

Sousa AAMorgan JTBrown PHAdams AJayasekara MPZhang GAckerson CJKruhlak MJLeapman RD
Small
2012 Jul 23

Xing RZhang FXie JAronova MZhang GGuo NHuang XSun XLiu GBryant LHBhirde ALiang AHou YLeapman RDSun SChen X
Nanoscale
2011 Dec

Bhirde AALiu GJin AIglesias-Bartolome RSousa AALeapman RDGutkind JSLee SChen X
Theranostics
2011

Cai THirai HZhang GZhang MTakahashi NKasai HSatin LSLeapman RDNotkins AL
Diabetologia
2011 Sep

Venzo AAntonello SGascón JAGuryanov ILeapman RDPerera NVSousa AZamuner MZanella AMaran F
Anal. Chem.
2011 Aug 15

Chen XNelson CDLi XWinters CAAzzam RSousa AALeapman RDGainer HSheng MReese TS
J. Neurosci.
2011 Apr 27

Xie JZhang FAronova MZhu LLin XQuan QLiu GZhang GChoi KYKim KSun XLee SSun SLeapman RChen X
ACS Nano
2011 Apr 26

Lin XXie JNiu GZhang FGao HYang MQuan QAronova MAZhang GLee SLeapman RChen X
Nano Lett.
2011 Feb 09

Aronova MASousa AALeapman RD
Micron
2011 Apr


Bhirde AAPatel SSousa AAPatel VMolinolo AAJi YLeapman RDGutkind JSRusling JF
Nanomedicine (Lond)
2010 Dec

Aronova MASousa AAZhang GLeapman RD
J Microsc
2010 Sep 01

Leapman RD
Nat Nanotechnol
2010 Jul

Fukunaga MLi TQvan Gelderen Pde Zwart JAShmueli KYao BLee JMaric DAronova MAZhang GLeapman RDSchenck JFMerkle HDuyn JH
Proc. Natl. Acad. Sci. U.S.A.
2010 Feb 23

Bhirde AASousa AAPatel VAzari AAGutkind JSLeapman RDRusling JF
Nanomedicine (Lond)
2009 Oct

Hohmann-Marriott MFSousa AAAzari AAGlushakova SZhang GZimmerberg JLeapman RD
Nat. Methods
2009 Oct

Chen KGLeapman RDZhang GLai BValencia JCCardarelli COVieira WDHearing VJGottesman MM
J. Natl. Cancer Inst.
2009 Sep 16

Sarin HKanevsky ASWu HSousa AAWilson CMAronova MAGriffiths GLLeapman RDVo HQ
J Transl Med
2009 Jun 23

Sousa AAAronova MAWu HSarin HGriffiths GLeapman RD
Nanomedicine (Lond)
2009 Jun

Paravastu AKQahwash ILeapman RDMeredith SCTycko R
Proc. Natl. Acad. Sci. U.S.A.
2009 May 05

Bhirde AAPatel VGavard JZhang GSousa AAMasedunskas ALeapman RDWeigert RGutkind JSRusling JF
ACS Nano
2009 Feb 24

Aronova MAKim YCPivovarova NBAndrews SBLeapman RD
Ultramicroscopy
2009 Feb


Sarin HKanevsky ASWu HBrimacombe KRFung SHSousa AAAuh SWilson CMSharma KAronova MALeapman RDGriffiths GLHall MD
J Transl Med
2008 Dec 18

Chen XWinters CAzzam RCrocker VLi XGalbraith JLeapman RReese Ts
Microsc. Microanal.
2008 Aug

Paravastu AKLeapman RDYau WMTycko R
Proc. Natl. Acad. Sci. U.S.A.
2008 Nov 25

Trachtenberg SDorward LMSperansky VVJaffe HAndrews SBLeapman RD
J. Mol. Biol.
2008 May 09




Chen XWinters CAzzam RLi XGalbraith JALeapman RDReese TS
Proc. Natl. Acad. Sci. U.S.A.
2008 Mar 18


Zhang GHirai HCai TMiura JYu PHuang HSchiller MRSwaim WDLeapman RDNotkins AL
J. Endocrinol.
2007 Nov


Aronova MAKim YCHarmon RSousa AAZhang GLeapman RD
J. Struct. Biol.
2007 Oct

Sousa AAAronova MAKim YCDorward LMZhang GLeapman RD
J. Struct. Biol.
2007 Sep

Daly MJGaidamakova EKMatrosova VYVasilenko AZhai MLeapman RDLai BRavel BLi SMKemner KMFredrickson JK
PLoS Biol.
2007 Apr

Leapman RDAronova MA
Methods Cell Biol.
2007

Chesnick IEAvallone FALeapman RDLandis WJEidelman NPotter K
Bone
2007 Apr

Aronova MAKim YCZhang GLeapman RD
Ultramicroscopy
2007

Chen KGValencia JCLai BZhang GPaterson JKRouzaud FBerens WWincovitch SMGarfield SHLeapman RDHearing VJGottesman MM
Proc. Natl. Acad. Sci. U.S.A.
2006 Jun 27

Chen XVinade LLeapman RDPetersen JDNakagawa TPhillips TMSheng MReese TS
Proc. Natl. Acad. Sci. U.S.A.
2005 Aug 09

Zhang PLand WLee SJuliani JLefman JSmith SRGermain DKessel MLeapman RRouault TASubramaniam S
J. Struct. Biol.
2005 May

Petkova ATLeapman RDGuo ZYau WMMattson MPTycko R
Science
2005 Jan 14


Petkova ATIshii YBalbach JJAntzutkin ONLeapman RDDelaglio FTycko R
Proc. Natl. Acad. Sci. U.S.A.
2002 Dec 24


Potter KLeapman RDBasser PJLandis WJ
J. Bone Miner. Res.
2002 Apr

Leapman RDRizzo NW
Ultramicroscopy
1999 Jun

Thirion STroadec JDPivovarova NBPagnotta SAndrews SBLeapman RDNicaise G
Proc. Natl. Acad. Sci. U.S.A.
1999 Mar 16

Leapman RDJarnik MSteven AC
J. Struct. Biol.
1997 Nov

Pozzo-Miller LDPivovarova NBLeapman RDBuchanan RAReese TSAndrews SB
J. Neurosci.
1997 Nov 15

Scott DADocampo RDvorak JAShi SLeapman RD
J. Biol. Chem.
1997 Oct 31



Shi SSun SAndrews SBLeapman RD
Microsc. Res. Tech.
1996 Feb 15


Silbermann MReddi AHHand ARLeapman RDVon der Mark KFranzen A
J. Anat.
1987 Apr

Andrews SBLeapman RDLandis DMReese TS
Proc. Natl. Acad. Sci. U.S.A.
1987 Mar

Livne EOliver CLeapman RDRosenberg LCPoole ARSilbermann M
J. Anat.
1987 Feb

Leapman RDGorlen KESwyt CR
Scan Electron Microsc
1985