Richard D Leapman, Ph.D.

portrait of Richard Leapman in lab
Scientific Director

Dr. Leapman obtained B.A. and M.A. in Natural Sciences, and Ph.D. in physics from the University of Cambridge, UK. He then trained as a postdoctoral fellow in the Department of Materials at the University of Oxford, UK, and in the Department of Applied and Engineering Physics at Cornell University, NY, where he contributed to the development of electron spectroscopy for the nanoscale characterization of materials.  He subsequently moved to NIH to develop methods based on scanning transmission electron microscopy and electron spectroscopy to determine the structure and chemical composition of cells and supramolecular assemblies. Dr. Leapman was elected a Fellow of the Microscopy Society of America in 2011.  He is currently Editor of the Journal of Microscopy (Oxford), a member of the editorial boards of other microscopy and nanotechnology journals, and has served on numerous national scientific advisory committees.  Dr. Leapman is Chief of NIBIB’s Laboratory of Cellular Imaging and Macromolecular Biophysics, while also serving as NIBIB’s Scientific Director since 2006.

Research Interests

The Section on Cellular and Supramolecular Structure and Function develops new methods based on electron microscopy and related techniques. Our aim is to expand knowledge about complex biological and disease processes, as well as to characterize morphologically the action of diagnostic markers and therapeutic agents in cells. The nanometer scale of biological electron microscopy lies between the realms of live-cell optical microscopy and atomic-scale structural tools of x-ray crystallography and NMR spectroscopy, both of which—unlike EM—require extraction and purification of cellular components. Our approach also complements cryo_EM of isolated frozen-hydrated supramolecular assemblies, which is difficult to apply to intact eukaryotic cells and tissues. We focus on the development of alternative quantitative imaging modes based on detection of elastic and inelastic electron scattering. Current research in the Section Includes development of techniques for (i) determining the tertiary and quaternary structures of macromolecular assemblies, (ii) visualizing 3D ultrastructure, (iii) mapping the elemental composition of subcellular compartments quantitatively, and (iv) studying bionanoparticles and their interactions with cells. We are applying these methods to structural biology, cellular biology, neurobiology, cancer biology, and nanomedicine.

Our group has been particularly active in developing the techniques of electron energy loss spectroscopy (EELS) and combining it with scanning transmission electron microscopy (STEM) to provide an unprecedented high spatial resolution for nanoanalysis of biological structures. We have devised new methods for quantifying both elemental and chemical information obtained from inelastic electron scattering. Recently, our group has extended the techniques of STEM and energy-filtered electron tomography to obtain three-dimensional structural and compositional information about cellular components.

Chen XCrosby KCFeng APurkey AMAronova MAWinters CACrocker VTLeapman RDReese TSDell'Acqua ML
Front Synaptic Neurosci

Rhee SWPokrovskaya IDBall KKLing KVedanaparti YCohen JCruz DRDZhao OSAronova MAZhang GKamykowski JALeapman RDStorrie B
Commun Biol
2021 Sep 16

Aronova MANoh SJZhang GByrnes CMeier ERKim YCLeapman RD
2021 Aug 20

Guay MDEmam ZASAnderson ABAronova MAPokrovskaya IDStorrie BLeapman RD
Sci Rep
2021 Jan 28

Pokrovskaya ITobin MDesai RAronova MAKamykowski JAZhang GJoshi SWhiteheart SWLeapman RDStorrie B
2021 Jul 04

Pokrovskaya IDTobin MDesai RJoshi SKamykowski JAZhang GAronova MAWhiteheart SWLeapman RDStorrie B
2021 Jan 02

McCauley JPPetroccione MAD'Brant LYTodd GCAffinnih NWisnoski JJZahid SShree SSousa AADe Guzman RMMigliore RBrazhe ALeapman RDKhmaladze ASemyanov AZuloaga DGMigliore MScimemi A
Cell Rep
2020 Oct 13

Rao AMcBride ELZhang GXu HCai TNotkins ALAronova MALeapman RD
J Struct Biol
2020 Oct 01

Pokrovskaya IDYadav SRao AMcBride EKamykowski JAZhang GAronova MALeapman RDStorrie B
Res Pract Thromb Haemost
2020 Jan

Chen XWinters CCrocker VLazarou MSousa AALeapman RDReese TS
Front Neuroanat

Pokrovskaya IDJoshi STobin MDesai RAronova MAKamykowski JAZhang GWhiteheart SWLeapman RDStorrie B
Blood Adv
2018 Nov 13

Shen ZSong JZhou ZYung BCAronova MALi YDai YFan WLiu YLi ZRuan HLeapman RDLin LNiu GChen XWu A
Adv Mater
2018 Jul 04

McBride ELRao AZhang GHoyne JDCalco GNKuo BCHe QPrince AAPokrovskaya IDStorrie BSousa AAAronova MALeapman RD
J Struct Biol
2018 Jun

Sweeney AMFleming KEMcCauley JPRodriguez MFMartin ETSousa AALeapman RDScimemi A
Sci Rep
2017 Mar 03

Lu NHuang PFan WWang ZLiu YWang SZhang GHu JLiu WNiu GLeapman RDLu GChen X
2017 May

Zhu LAlmaça JDadi PKHong HSakamoto WRossi MLee RJVierra NCLu HCui YMcMillin SMPerry NAGurevich VVLee AKuo BLeapman RDMatschinsky FMDoliba NMUrs NMCaron MGJacobson DACaicedo AWess J
Nat Commun
2017 Feb 01

Yadav SWilliamson JKAronova MAPrince AAPokrovskaya IDLeapman RDStorrie B
2017 Jun

Bryant LHKim SJHobson MMilo BKovacs ZIJikaria NLewis BKAronova MASousa AAZhang GLeapman RDFrank JA
2017 Feb

Guay MDCzaja WAronova MALeapman RD
Sci Rep
2016 Jun 13

Zhu GLiu YYang XKim YHZhang HJia RLiao HSJin ALin JAronova MLeapman RNie ZNiu GChen X
2016 Mar 28

Sousa AAHassan SAKnittel LLBalbo AAronova MABrown PHSchuck PLeapman RD
2016 Mar 28

Pokrovskaya IDAronova MAKamykowski JAPrince AAHoyne JDCalco GNKuo BCHe QLeapman RDStorrie B
J Thromb Haemost
2016 Mar

Chen XLevy JMHou AWinters CAzzam RSousa AALeapman RDNicoll RAReese TS
Proc Natl Acad Sci U S A
2015 Dec 15

Chen RJZhang GGarfield SHShi YJChen KGRobey PGLeapman RD
PLoS One

Pfeifer CRShomorony AAronova MAZhang GCai TXu HNotkins ALLeapman RD
J Struct Biol
2015 Jan

Pothayee NChen DYAronova MAQian CBouraoud NDodd SLeapman RDKoretsky AP
J Mater Chem B

Graydon CWZhang JOesch NWSousa AALeapman RDDiamond JS
J Neurosci
2014 Jul 02

Bhirde AAChikkaveeraiah BVSrivatsan ANiu GJin AJKapoor AWang ZPatel SPatel VGorbach AMLeapman RDGutkind JSHight Walker ARChen X
ACS Nano
2014 May 27

Huang PLin JLi WRong PWang ZWang SWang XSun XAronova MNiu GLeapman RDNie ZChen X
Angew Chem Int Ed Engl
2013 Dec 23

Abreu FSousa AAAronova MAKim YCox DLeapman RDAndrade LRKachar BBazylinski DALins U
J Struct Biol
2013 Feb

Norlin NHellberg MFilippov ASousa AAGröbner GLeapman RDAlmqvist NAntzutkin ON
J Struct Biol
2012 Oct

Fera ADosemeci ASousa AAYang CLeapman RDReese TS
J Comp Neurol
2012 Dec 15

Bhirde AAKapoor ALiu GIglesias-Bartolome RJin AZhang GXing RLee SLeapman RDGutkind JSChen X
ACS Nano
2012 Jun 26

Sousa AAMorgan JTBrown PHAdams AJayasekara MPZhang GAckerson CJKruhlak MJLeapman RD
2012 Jul 23

Xing RZhang FXie JAronova MZhang GGuo NHuang XSun XLiu GBryant LHBhirde ALiang AHou YLeapman RDSun SChen X
2011 Dec

Bhirde AALiu GJin AIglesias-Bartolome RSousa AALeapman RDGutkind JSLee SChen X

Cai THirai HZhang GZhang MTakahashi NKasai HSatin LSLeapman RDNotkins AL
2011 Sep

Venzo AAntonello SGascón JAGuryanov ILeapman RDPerera NVSousa AZamuner MZanella AMaran F
Anal Chem
2011 Aug 15

Chen XNelson CDLi XWinters CAAzzam RSousa AALeapman RDGainer HSheng MReese TS
J Neurosci
2011 Apr 27

Xie JZhang FAronova MZhu LLin XQuan QLiu GZhang GChoi KYKim KSun XLee SSun SLeapman RChen X
ACS Nano
2011 Apr 26

Lin XXie JNiu GZhang FGao HYang MQuan QAronova MAZhang GLee SLeapman RChen X
Nano Lett
2011 Feb 09

Aronova MASousa AALeapman RD
2011 Apr

Bhirde AAPatel SSousa AAPatel VMolinolo AAJi YLeapman RDGutkind JSRusling JF
Nanomedicine (Lond)
2010 Dec

Aronova MASousa AAZhang GLeapman RD
J Microsc
2010 Sep 01

Leapman RD
Nat Nanotechnol
2010 Jul

Fukunaga MLi TQvan Gelderen Pde Zwart JAShmueli KYao BLee JMaric DAronova MAZhang GLeapman RDSchenck JFMerkle HDuyn JH
Proc Natl Acad Sci U S A
2010 Feb 23

Bhirde AASousa AAPatel VAzari AAGutkind JSLeapman RDRusling JF
Nanomedicine (Lond)
2009 Oct

Hohmann-Marriott MFSousa AAAzari AAGlushakova SZhang GZimmerberg JLeapman RD
Nat Methods
2009 Oct

Chen KGLeapman RDZhang GLai BValencia JCCardarelli COVieira WDHearing VJGottesman MM
J Natl Cancer Inst
2009 Sep 16

Sarin HKanevsky ASWu HSousa AAWilson CMAronova MAGriffiths GLLeapman RDVo HQ
J Transl Med
2009 Jun 23

Sousa AAAronova MAWu HSarin HGriffiths GLeapman RD
Nanomedicine (Lond)
2009 Jun

Paravastu AKQahwash ILeapman RDMeredith SCTycko R
Proc Natl Acad Sci U S A
2009 May 05

Bhirde AAPatel VGavard JZhang GSousa AAMasedunskas ALeapman RDWeigert RGutkind JSRusling JF
ACS Nano
2009 Feb 24

Aronova MAKim YCPivovarova NBAndrews SBLeapman RD
2009 Feb

Sarin HKanevsky ASWu HBrimacombe KRFung SHSousa AAAuh SWilson CMSharma KAronova MALeapman RDGriffiths GLHall MD
J Transl Med
2008 Dec 18

Chen XWinters CAzzam RCrocker VLi XGalbraith JLeapman RReese Ts
Microsc Microanal
2008 Aug

Paravastu AKLeapman RDYau WMTycko R
Proc Natl Acad Sci U S A
2008 Nov 25

Trachtenberg SDorward LMSperansky VVJaffe HAndrews SBLeapman RD
J Mol Biol
2008 May 09

Chen XWinters CAzzam RLi XGalbraith JALeapman RDReese TS
Proc Natl Acad Sci U S A
2008 Mar 18

Zhang GHirai HCai TMiura JYu PHuang HSchiller MRSwaim WDLeapman RDNotkins AL
J Endocrinol
2007 Nov

Sousa AAAronova MAKim YCDorward LMZhang GLeapman RD
J Struct Biol
2007 Sep

Daly MJGaidamakova EKMatrosova VYVasilenko AZhai MLeapman RDLai BRavel BLi SMKemner KMFredrickson JK
PLoS Biol
2007 Apr

Chesnick IEAvallone FALeapman RDLandis WJEidelman NPotter K
2007 Apr

Aronova MAKim YCZhang GLeapman RD

Chen KGValencia JCLai BZhang GPaterson JKRouzaud FBerens WWincovitch SMGarfield SHLeapman RDHearing VJGottesman MM
Proc Natl Acad Sci U S A
2006 Jun 27

Chen XVinade LLeapman RDPetersen JDNakagawa TPhillips TMSheng MReese TS
Proc Natl Acad Sci U S A
2005 Aug 09

Zhang PLand WLee SJuliani JLefman JSmith SRGermain DKessel MLeapman RRouault TASubramaniam S
J Struct Biol
2005 May

Petkova ATLeapman RDGuo ZYau WMMattson MPTycko R
2005 Jan 14

Petkova ATIshii YBalbach JJAntzutkin ONLeapman RDDelaglio FTycko R
Proc Natl Acad Sci U S A
2002 Dec 24

Potter KLeapman RDBasser PJLandis WJ
J Bone Miner Res
2002 Apr

Leapman RDRizzo NW
1999 Jun

Thirion STroadec JDPivovarova NBPagnotta SAndrews SBLeapman RDNicaise G
Proc Natl Acad Sci U S A
1999 Mar 16

Leapman RDJarnik MSteven AC
J Struct Biol
1997 Nov

Pozzo-Miller LDPivovarova NBLeapman RDBuchanan RAReese TSAndrews SB
J Neurosci
1997 Nov 15

Scott DADocampo RDvorak JAShi SLeapman RD
J Biol Chem
1997 Oct 31

Shi SSun SAndrews SBLeapman RD
Microsc Res Tech
1996 Feb 15

Silbermann MReddi AHHand ARLeapman RDVon der Mark KFranzen A
J Anat
1987 Apr

Andrews SBLeapman RDLandis DMReese TS
Proc Natl Acad Sci U S A
1987 Mar

Livne EOliver CLeapman RDRosenberg LCPoole ARSilbermann M
J Anat
1987 Feb

Leapman RDGorlen KESwyt CR
Scan Electron Microsc