Capable of unsurpassed optical resolution, this system is a clone of the original prototype developed at the Janelia Research Campus, but offers 3 color capability. IPALM combines photoactivated localization microscopy with single-photon, simultaneous multiphase interferometry providing <20 nm resolution in all three dimensions. This microscope is currently located in the Taraska Lab and will move to AIM once the facility is operational.
Donating lab: Justin Taraska, NHLBI
Spatial resolution: 20nm in X, Y. 10nm in Z.
Temporal resolution: Fixed samples only.
Excitation wavelengths: 488nm, 647nm, 750nm.
Detection channels: Single channel (3 cameras used for interferometric localization of single emitters). Multiple emission bands are acquired sequentially.
Sample specifications: Fixed samples sandwiched between two coverslips <10 microns apart. The sample is placed between two objectives in a 4Pi geometry and images can be acquired up to 750nm from the coverslip surface. This is ideally suited for high resolution imaging of membrane or peri-membrane structures.
- Highest resolution optical microscope currently available.
- Provides unparalleled axial resolution.
- Difficult sample preparation protocol.
- Sample thickness is restricted and only sub-volumes of mammalian cells can be imaged.
Shtengel et al. PNAS 106(9): 3125-3130, Mar 2009.