Instant Structured Illumination Microscope (ISIM)/ Total Internal Reflection Fluorescence SIM (TIRF-SIM)

Share:

EmailFacebookLinkedInXWhatsAppShare

The ISIM is a fluorescence microscope that combines rapid image acquisition (100Hz) with a resolution roughly double that of a confocal microscope in all three dimensions. Unlike other structured illumination microscopes, the resolution is enhanced via hardware in a single frame acquisition rather than computationally processing multiple images, effectively making this an ‘instantaneous’ structured illumination microscope. Recently, the Shroff Lab has extended the capability of this microscope to rapid, super-resolution TIRF.  

Spatial resolution: 140nm lateral, 350nm axial

Temporal resolution: Framerate up to 100Hz

Excitation wavelengths: 488nm, 561nm and 639nm

Image Gallery

Instant TIRF-SIM instrument schematic

Figure 1: Instant TIRF-SIM instrument schematic

 

ER dynamics at 100 Hz​​

Figure 2: ER dynamics at 100 Hz​​

 

HRAS dynamics revealed by TIRF-SIM

Figure 3. HRAS dynamics revealed by TIRF-SIM

 

Video Gallery

{"preview_thumbnail":"/sites/default/files/styles/video_embed_wysiwyg_preview/public/video_thumbnails/GzAtogrKHBU.jpg?itok=ksRbA5wB","video_url":"https://youtu.be/GzAtogrKHBU","settings":{"responsive":1,"width":"854","height":"480","autoplay":0},"settings_summary":["Embedded Video (Responsive)."]}

{"preview_thumbnail":"/sites/default/files/styles/video_embed_wysiwyg_preview/public/video_thumbnails/a4hYbHiZwsA.jpg?itok=j7qOS0Qi","video_url":"https://youtu.be/a4hYbHiZwsA","settings":{"responsive":1,"width":"854","height":"480","autoplay":0},"settings_summary":["Embedded Video (Responsive)."]}

References:

1. Instant super-resolution imaging in live cells and embryos via analog image processing. Nat Methods. 2013 Nov;10(11):1122-6.

2.  Single-shot super-resolution total internal reflection fluorescence microscopy. Nat Methods. 2018 Jun;15(6):425-428.

EmailFacebookLinkedInXWhatsAppShare