High throughput single molecule imaging microscope

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The high throughput single molecule microscope was designed to image large numbers of cell samples in a multi-well format. This microscope can routinely image up to 8 coverslips and 10,000+ cells per acquisition. An included analysis pipeline automatically segments images to identify nuclei, cell bodies, and puncta (RNAs, receptors, etc.) and generates statistics on these puncta (number/cell, co-expression, co-localization, etc.). This microscope is currently located in the Larson Lab and will move to AIM once the facility is operational.

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High throughput single molecule FISH
Figure 1. High throughput single molecule FISH

 

Example of pipeline for single-molecule RNA FISH Analysis
Figure 2. Pipeline for single-molecule RNA FISH Analysis
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