Scanning Probe Microscopy Unit

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The Scanning Probe Microscopy Unit of the BEPS Shared Resource specializes in the following:

  • Sub-nm resolution imaging of molecular complexes, supported lipid bilayers, cells, and tissues
  • Molecular recognition, protein unfolding
  • Force spectroscopy, visco-elastic properties
  • Simultaneous, co-localized AFM and fluorescence (including TIRF and confocal) microscopy
  • Mathematical modeling, finite element analysis

We are located in Building 13 on the NIH campus.

Atomic Force Imaging and Spectroscopy

AFM:

  • Sub-nm resolution imaging of macromolecular complexes (including protein-protein, protein-DNA, and/or RNA), supported lipid bilayers, cells and tissues under ambient conditions
  • Force spectroscopy:  nano-indentation strategies to map viscoelastic, adhesion and other properties at nm resolution (including single molecules, lipid bilayers, live cells and tissues)
  • Studies of protein unfolding

Molecular Recognition: Antigen recognition by binding events with antibody-functionalized probes. Immuno-AFM: Molecular recognition of protein-antibody complexes by direct AFM imaging. Co-localized immuno-fluorescence and immuno-AFM.

Mathematical Modeling: Mathematical modeling of biophysical systems, image analysis methodologies, finite element analysis.

Auditory Mechanics:

Project examples

Nucleus mechanical properties of HMGN5 over-expressing cells (Dr. M. Bustin, CCR, NCI)

Microscopic image of a cell and a figure with results next to it

Cell visco-elastic properties were measured on live cells with the AFM

Novel DNA-RNA complexes (Dr. S. Adhya, CCR, NCI)

Novel bacterial RNA binds and bridges DNA double-strands

 

 

 

 

 

 

Novel bacterial RNA binds and bridges DNA double-strands

Zhang LMuirhead KJSyed ZADimitriadis EKTen Hagen KG
Proc Natl Acad Sci U S A
2024 Feb 06

Carvajal-Barriga EJFitzgerald WDimitriadis EKMargolis LFields RD
Sci Rep
2023 Apr 28

Barriga EJCFitzgerald WDimitriadis EKMargolis LFields RD
Res Sq
2022 Oct 28

Son BPatterson-West JArroyo-Mendoza MRamachandran RIben JRZhu JRao VDimitriadis EKHinton DM
Nucleic Acids Res
2021 Sep 20

Varghese PKAbu-Asab MDimitriadis EKDolinska MBMorcos GPSergeev YV
Int J Mol Sci
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Melters DPPitman MRakshit TDimitriadis EKBui MPapoian GADalal Y
Proc Natl Acad Sci U S A
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DuChez BJDoyle ADDimitriadis EKYamada KM
Biophys J
2019 Feb 19

Kus NJDolinska MBYoung KLDimitriadis EKWingfield PTSergeev YV
PLoS One
2018

Jin XDimitriadis EKLiu YCombs CAChang JVarsano NStempinski EFlores RJackson SNMuller LWoods ASAddadi LKruth HS
Arterioscler Thromb Vasc Biol
2018 Jul


Hategan ABianchet MASteiner JKarnaukhova EMasliah EFields ALee MHDickens AMHaughey NDimitriadis EKNath A
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Qian ZMacvanin MDimitriadis EKHe XZhurkin VAdhya S
mBio
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J Cell Biol
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Furusawa TRochman MTaher LDimitriadis EKNagashima KAnderson SBustin M
Nat Commun
2015 Jan 22

Chandran PLDimitriadis EKLisziewicz JSperansky VHorkay F
Soft Matter
2014 Oct 14

Walkiewicz MPDimitriadis EKDalal Y
Nat Struct Mol Biol
2014 Jan

Bui MDimitriadis EKHoischen CAn EQuénet DGiebe SNita-Lazar ADiekmann SDalal Y
Cell
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Shukla HVaitiekunas PMajumdar AKDragan AIDimitriadis EKKotova SCrane-Robinson CPrivalov PL
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Chandran PLDimitriadis EKBasser PJHorkay F
J Polym Sci B Polym Phys
2010 Dec 15

Dimitriadis EKWeber CGill RKDiekmann SDalal Y
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Kotova SVijayasarathy CDimitriadis EKIkonomou LJaffe HSieving PA
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Sinha GPSabri FDimitriadis EKIwasa KH
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Grundy GJRamón-Maiques SDimitriadis EKKotova SBiertümpfel CHeymann JBSteven ACGellert MYang W
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Manoussaki DChadwick RSKetten DRArruda JDimitriadis EKO'Malley JT
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Roy SDimitriadis EKKar SGeanacopoulos MLewis MSAdhya S
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