1. Labs @ NIBIB
  2. Advanced Imaging and Microscopy (AIM) Resource

Advanced Imaging and Microscopy (AIM) Resource

The Advanced Imaging & Microscopy Resource is a trans-NIH shared resource that houses, operates, disseminates, and improves non-commercial, prototype optical imaging systems developed at the NIH. The facilities at AIM are available for use by the entire NIH intramural research community.

While we welcome users with any size imaging project, AIM specializes in large, year long (or longer), collaborative research efforts with NIH labs – providing sample preparation, microscope operation and development, and data processing/analysis as needed. We take care of the imaging and processing so that you can focus on the biology.

Lab Resources

Microscope Description Spatial Resolution Speed
(frames/s)		 Photodose Sample

Instant structured illumination microscope (ISIM)

Fast super-resolution

140nm lateral
350nm axial

 100

High

Live or fixed. Small, thin samples like cells, organoids.

Dual-view Inverted Selective Plane Illumination Microscope (DISPIM)

Fast, gentle, high resolution lightsheet for live samples

330nm isotropic

 100

Low

Live or fixed. Cells, up to medium size, transparent samples like organoids, C. elegans, zebrafish.

Cleared Tissue DISPIM

Fast multi-view lightsheet for large cleared tissue samples

500nm isotropic

 100

Low

Fixed and cleared. Up to 5mm thick slabs of tissue, up to 2cm x 3cm lateral size.

Single Molecule TIRF/Inclined Illumination Microscope

Variable inclination illumination single molecule imaging. Including STORM. 

20 nm lateral
50-60 nm axial

 100

High

Live or fixed. Small, thin samples like cells.

Interferometric Photoactivated Localization Microscope (IPALM)

Localization microscope with unprecedented lateral and axial resolution.

20 nm lateral
10nm axial

Not suitable for live cells

High

Fixed and specially mounted. Small and thin samples like cells, bacteria.

This table describes the spatial resolution, speed, and sample suitability of the five microscopes currently being operated by the Advanced Imaging and Microscopy (AIM) Resource.

 

 

Additional Lab Resource Information: 

Staff

Publications

Targeting N-linked Glycosylation for the Therapy of Aggressive Lymphomas.
Scheich SChen JLiu JSchnutgen FEnssle JCCeribelli MThomas CJChoi JMorris VHsiao TNguyen HWang BBolomsky APhelan JDCorcoran SUrlaub HYoung RMHaupl BWright GWHuang DWJi YYu XXu WYang YZhao HMuppidi JPan KTOellerich TStaudt LM
Cancer Discov
2023 May 04

A conserved oligomerization domain in the disordered linker of coronavirus nucleocapsid proteins.
Zhao HWu DHassan SANguyen AChen JPiszczek GSchuck P
Sci Adv
2023 Apr 05

Label-free cleared tissue microscopy and machine learning for 3D histopathology of biomaterial implants.
Ngo TBDeStefano SLiu JSu YShroff HVishwasrao HDSadtler K
J Biomed Mater Res A
2023 Jun

Three-dimensional structured illumination microscopy with enhanced axial resolution.
Li XWu YSu YRey-Suarez IMatthaeus CUpdegrove TBWei ZZhang LSasaki HLi YGuo MGiannini JPVishwasrao HDChen JLee SJShao LLiu HRamamurthi KSTaraska JWUpadhyaya ALa Riviere PShroff H
Nat Biotechnol
2023 Jan 26

Incorporating the image formation process into deep learning improves network performance.
Li YSu YGuo MHan XLiu JVishwasrao HDLi XChristensen RSengupta TMoyle MWRey-Suarez IChen JUpadhyaya AUsdin TBColón-Ramos DALiu HWu YShroff H
Nat Methods
2022 Nov

Practical considerations for quantitative light sheet fluorescence microscopy.
Hobson CMGuo MVishwasrao HDWu YShroff HChew TL
Nat Methods
2022 Dec

Plasticity in structure and assembly of SARS-CoV-2 nucleocapsid protein.
Zhao HNguyen AWu DLi YHassan SAChen JShroff HPiszczek GSchuck P
PNAS Nexus
2022 May

[Analysis of Pollution Characteristics and Sources of Rainfall Runoff from Roofs in the Central District of Beijing].
Xi YGuo JTao LTian YChen JJWu YXu SSJing HWLiu BX
Huan Jing Ke Xue
2022 Jun 08

The HIV-1 Viral Protease Is Activated during Assembly and Budding Prior to Particle Release.
Tabler COWegman SJChen JShroff HAlhusaini NTilton JC
J Virol
2022 May 11

Plasticity in structure and assembly of SARS-CoV-2 nucleocapsid protein.
Zhao HNguyen AWu DLi YHassan SAChen JShroff HPiszczek GSchuck P
bioRxiv
2022 Feb 09

Multiview confocal super-resolution microscopy.
Wu YHan XSu YGlidewell MDaniels JSLiu JSengupta TRey-Suarez IFischer RPatel ACombs CSun JWu XChristensen RSmith CBao LSun YDuncan LHChen JPommier YShi YBMurphy ERoy SUpadhyaya AColón-Ramos DLa Riviere PShroff H
Nature
2021 Dec

PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation.
Sun YChen JHuang SNSu YPWang WAgama KSaha SJenkins LMPascal JMPommier Y
Nat Commun
2021 Aug 18

Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes.
Chen JSasaki HLai HSu YLiu JWu YZhovmer ACombs CARey-Suarez IChang HYHuang CCLi XGuo MNizambad SUpadhyaya ALee SJLucas LAGShroff H
Nat Methods
2021 Jun

A polymer index-matched to water enables diverse applications in fluorescence microscopy.
Han XSu YWhite HO'Neill KMMorgan NYChristensen RPotarazu DVishwasrao HDXu SSun YHuang SYMoyle MWDai QPommier YGiniger EAlbrecht DRProbst RShroff H
Lab Chip
2021 Apr 20

Semi-automated single-molecule microscopy screening of fast-dissociating specific antibodies directly from hybridoma cultures.
Miyoshi TZhang QMiyake TWatanabe SOhnishi HChen JVishwasrao HDChakraborty OBelyantseva IAPerrin BJShroff HFriedman TBOmori KWatanabe N
Cell Rep
2021 Feb 02

Zebrafish Posterior Lateral Line primordium migration requires interactions between a superficial sheath of motile cells and the skin.
Dalle Nogare DENatesh NVishwasrao HDShroff HChitnis AB
Elife
2020 Nov 25

[Concentration and Carbon Isotope Composition of DOC and DIC in the Miyun Reservoir Watershed in Summer].
Chen JJGuo JXu SSTao LJing HW
Huan Jing Ke Xue
2020 Nov 08

Quantum Dot-Conjugated SARS-CoV-2 Spike Pseudo-Virions Enable Tracking of Angiotensin Converting Enzyme 2 Binding and Endocytosis.
Gorshkov KSusumu KChen JXu MPradhan MZhu WHu XBreger JCWolak MOh E
ACS Nano
2020 Sep 22

Rapid image deconvolution and multiview fusion for optical microscopy.
Guo MLi YSu YLambert TNogare DDMoyle MWDuncan LHIkegami RSantella ARey-Suarez IGreen DBeiriger AChen JVishwasrao HGanesan SPrince VWaters JCAnnunziata CMHafner MMohler WAChitnis ABUpadhyaya AUsdin TBBao ZColón-Ramos DLa Riviere PLiu HWu YShroff H
Nat Biotechnol
2020 Nov

Visualizing the translation and packaging of HIV-1 full-length RNA.
Chen JLiu YWu BNikolaitchik OAMohan PRChen JPathak VKHu WS
Proc Natl Acad Sci U S A
2020 Mar 17

Histone hyperacetylation disrupts core gene regulatory architecture in rhabdomyosarcoma.
Gryder BEPomella SSayers CWu XSSong YChiarella AMBagchi SChou HCSinniah RSWalton AWen XRota RHathaway NAZhao KChen JVakoc CRShern JFStanton BZKhan J
Nat Genet
2019 Dec

A 2-dimensional ratchet model describes assembly initiation of a specialized bacterial cell surface.
Peluso EAUpdegrove TBChen JShroff HRamamurthi KS
Proc Natl Acad Sci U S A
2019 Oct 22

In situ polymerization on nanoscale metal-organic frameworks for enhanced physiological stability and stimulus-responsive intracellular drug delivery.
Liu YGong CSDai YYang ZYu GLiu YZhang MLin LTang WZhou ZZhu GChen JJacobson OKiesewetter DOWang ZChen X
Biomaterials
2019 Oct

Gas chromatography-mass spectrometry based metabolomics profile of hippocampus and cerebellum in mice after chronic arsenic exposure.
Mo TTDai HDu HZhang RYChai KPAn YChen JJWang JKChen ZJChen CZJiang XJTang RWang LPTan QTang PMiao XYMeng PZhang LBCheng SQPeng BTu BJHan TLXia YYBaker PN
Environ Toxicol
2019 Feb

[Nitrous Oxide Production in Response to Oxygen in a Solar Greenhouse Vegetable Soil].
Chen JJSong HCao WCWang YRWang JG
Huan Jing Ke Xue
2018 Aug 08

Single-shot super-resolution total internal reflection fluorescence microscopy.
Guo MChandris PGiannini JPTrexler AJFischer RChen JVishwasrao HDRey-Suarez IWu YWu XWaterman CMPatterson GHUpadhyaya ATaraska JWShroff H
Nat Methods
2018 Jun

A RAS-CaMKKβ-AMPKα2 pathway promotes senescence by licensing post-translational activation of C/EBPβ through a novel 3'UTR mechanism.
Basu SKGonit MSalotti JChen JBhat AGorospe MViollet BClaffey KPJohnson PF
Oncogene
2018 Jun

Albumin/vaccine nanocomplexes that assemble in vivo for combination cancer immunotherapy.
Zhu GLynn GMJacobson OChen KLiu YZhang HMa YZhang FTian RNi QCheng SWang ZLu NYung BCWang ZLang LFu XJin AWeiss IDVishwasrao HNiu GShroff HKlinman DMSeder RAChen X
Nat Commun
2017 Dec 05

Intertwining DNA-RNA nanocapsules loaded with tumor neoantigens as synergistic nanovaccines for cancer immunotherapy.
Zhu GMei LVishwasrao HDJacobson OWang ZLiu YYung BCFu XJin ANiu GWang QZhang FShroff HChen X
Nat Commun
2017 Nov 14

Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy.
Wu YKumar ASmith CArdiel EChandris PChristensen RRey-Suarez IGuo MVishwasrao HDChen JTang JUpadhyaya ALa Riviere PJShroff H
Nat Commun
2017 Nov 13

DNA-RNA interactions are critical for chromosome condensation in .
Qian ZZhurkin VBAdhya S
Proc Natl Acad Sci U S A
2017 Nov 14

Myc Regulates Chromatin Decompaction and Nuclear Architecture during B Cell Activation.
Kieffer-Kwon KRNimura KRao SSPXu JJung SPekowska ADose MStevens EMathe EDong PHuang SCRicci MABaranello LZheng YTomassoni Ardori FResch WStavreva DNelson SMcAndrew MCasellas AFinn EGregory CSt Hilaire BGJohnson SMDubois WCosma MPBatchelor ELevens DPhair RDMisteli TTessarollo LHager GLakadamyali MLiu ZFloer MShroff HAiden ELCasellas R
Mol Cell
2017 Aug 17

Real-time visualization of chromatin modification in isolated nuclei.
Sardo LLin AKhakhina SBeckman LRicon LElbezanti WJaison TVishwasrao HShroff HJanetopoulos CKlase ZA
J Cell Sci
2017 Sep 01

Probing the target search of DNA-binding proteins in mammalian cells using TetR as model searcher.
Normanno DBoudarène LDugast-Darzacq CChen JRichter CProux FBénichou OVoituriez RDarzacq XDahan M
Nat Commun
2015 Jul 07

A general method to improve fluorophores for live-cell and single-molecule microscopy.
Grimm JBEnglish BPChen JSlaughter JPZhang ZRevyakin APatel RMacklin JJNormanno DSinger RHLionnet TLavis LD
Nat Methods
2015 Mar

Whole-cell, multicolor superresolution imaging using volumetric multifocus microscopy.
Hajj BWisniewski JEl Beheiry MChen JRevyakin AWu CDahan M
Proc Natl Acad Sci U S A
2014 Dec 09

Imaging the fate of histone Cse4 reveals de novo replacement in S phase and subsequent stable residence at centromeres.
Wisniewski JHajj BChen JMizuguchi GXiao HWei DDahan MWu C
Elife
2014 May 20

Single-molecule dynamics of enhanceosome assembly in embryonic stem cells.
Chen JZhang ZLi LChen BCRevyakin AHajj BLegant WDahan MLionnet TBetzig ETjian RLiu Z
Cell
2014 Mar 13